TNNI1 Human

Troponin I Type 1 Human Recombinant
Cat. No.
BT16766
Source
Escherichia Coli.
Synonyms
DKFZp451O223, SSTNI, TNN1, Troponin I, slow skeletal muscle ,Troponin I, slow-twitch isoform.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TNNI1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 207 amino acids (1-187 a.a.) and having a molecular mass of 23.8kDa.TNNI1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Troponin I, also known as TNNI1, belongs to the troponin I protein family. The troponin complex comprises three subunits, with TNNI1 acting as the inhibitory subunit. This subunit plays a crucial role in muscle relaxation by preventing the interaction between actin and myosin in striated muscles. TNNI1 achieves this by binding to tropomyosin, which allows it to control the sensitivity of striated muscles to calcium. This regulation is achieved through structural changes within the actin-myosin complexes.
Description
Recombinant human TNNI1, produced in E. coli, is a single polypeptide chain that lacks glycosylation. It consists of 207 amino acids, spanning from position 1 to 187, and possesses a molecular weight of 23.8 kDa. A 20-amino acid His-tag is fused to the N-terminus of TNNI1 to facilitate purification using specialized chromatographic methods.
Physical Appearance
A clear solution that has been sterilized through filtration.
Formulation
The TNNI1 protein solution has a concentration of 1 mg/ml and is prepared in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.4 M urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein such as HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of the protein is determined using SDS-PAGE analysis and is confirmed to be greater than 90.0%.
Synonyms
DKFZp451O223, SSTNI, TNN1, Troponin I, slow skeletal muscle ,Troponin I, slow-twitch isoform.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MPEVERKPKI TASRKLLLKS LMLAKAKECW EQEHEEREAE KVRYLAERIP TLQTRGLSLS ALQDLCRELH AKVEVVDEER YDIEAKCLHN TREIKDLKLK VMDLRGKFKR PPLRRVRVSA DAMLRALLGS KHKVSMDLRA NLKSVKKEDT EKERPVEVGD WRKNVEAMSG MEGRKKMFDA AKSPTSQ

Product Science Overview

Expression and Function

Troponin I Type 1 is primarily expressed in skeletal muscle and functions to prevent actin-myosin interaction, which is crucial for muscle relaxation. It is also considered a cytoskeletal element in some neurons . The recombinant form of this protein is produced using Escherichia coli (E. coli) expression systems, which allows for high purity and suitability for various applications such as SDS-PAGE .

Importance in Muscle Development

TNNI1 is specifically expressed in slow muscle fibers and plays a significant role in muscle development. It is involved in the regulation of muscle contraction by inhibiting the interaction between actin and myosin, which is essential for muscle relaxation . This regulation is crucial for maintaining proper muscle function and preventing unwanted contractions.

Applications and Research

Recombinant Human Troponin I Type 1 is widely used in research to study muscle physiology and the mechanisms of muscle contraction and relaxation. It is also used in the development of diagnostic assays for muscle-related diseases and conditions. The high purity and specific expression of recombinant TNNI1 make it a valuable tool for researchers studying muscle function and related disorders .

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