TNNI1 Paired Antibody
DKFZp451O223, SSTNI, TNN1, Troponin I, slow skeletal muscle ,Troponin I, slow-twitch isoform.
2 vials of sterile Filtered clear colorless solution.
Greater than 95%.
Description
TNNI1 gold conjugation antibody and TNNI1 capture antibody are used to develop rapid test for TNNI1 rapid test. Please note that when ordering for example: 50µg antibody we ship 25µg from each of the antibodies (50µg in total).
Product Specs
DKFZp451O223, SSTNI, TNN1, Troponin I, slow skeletal muscle ,Troponin I, slow-twitch isoform.
Purified monoclonal IgG by protein A chromatography.
Product Science Overview
Mouse anti-human Troponin I Type 1 paired antibodies are monoclonal antibodies specifically designed to target and bind to human Troponin I Type 1. These antibodies are widely used in research and diagnostic applications, particularly in the study of muscle physiology and cardiac diseases.
Troponin I is a part of the troponin complex, which is essential for muscle contraction in skeletal and cardiac muscles. The troponin complex consists of three subunits:
- Troponin C (TnC): Binds calcium ions.
- Troponin T (TnT): Binds to tropomyosin, anchoring the troponin complex.
- Troponin I (TnI): Inhibits actomyosin ATPase, preventing muscle contraction in the absence of calcium.
Troponin I Type 1 (TNNI1) is specifically expressed in slow-twitch skeletal muscle fibers. It plays a crucial role in muscle relaxation by inhibiting the interaction between actin and myosin .
Monoclonal antibodies are produced by identical immune cells that are clones of a unique parent cell. They have high specificity for a particular antigen, making them valuable tools in research and diagnostics. Mouse monoclonal antibodies are generated by immunizing mice with the target antigen, followed by the fusion of spleen cells with myeloma cells to create hybridomas. These hybridomas are then screened for the production of the desired antibody .
The preparation of mouse anti-human Troponin I Type 1 paired antibodies involves several steps:
- Immunization: Mice are immunized with human Troponin I Type 1 protein or peptide to elicit an immune response.
- Cell Fusion: Spleen cells from the immunized mice are fused with myeloma cells to create hybridomas.
- Screening: Hybridomas are screened for the production of antibodies that specifically bind to human Troponin I Type 1.
- Cloning: Positive hybridomas are cloned to ensure the production of monoclonal antibodies.
- Purification: The antibodies are purified from the hybridoma culture supernatant using techniques such as protein A/G affinity chromatography .
Mouse anti-human Troponin I Type 1 paired antibodies are used in various applications, including:
