TNNI1 Antibody

Troponin I Type 1, Mouse Anti Human
Cat. No.
BT1656
Source
Synonyms
DKFZp451O223, SSTNI, TNN1, Troponin I, slow skeletal muscle ,Troponin I, slow-twitch isoform.
Appearance
Sterile Filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Product Specs

Introduction
Troponin I (TNNI1), a member of the troponin I family, constitutes one subunit of the three-part troponin complex. This inhibitory subunit prevents actin-myosin interaction, playing a crucial role in the relaxation of striated muscles. By complexing with tropomyosin, TNNI1 regulates the calcium sensitivity of striated muscles through structural modifications within the actin-myosin complex.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The antibody is supplied at a concentration of 1mg/ml in a buffer consisting of phosphate-buffered saline (PBS) at pH 7.4, 10% glycerol, and 0.01% sodium azide.
Storage Procedures
Store the antibody at 4°C for up to one month. For long-term storage, store at -20°C. Avoid repeated freeze-thaw cycles to maintain stability.
Stability / Shelf Life
The antibody has a shelf life of 12 months when stored at -20°C and one month when stored at 4°C.
Applications
This antibody has been rigorously tested for specificity and reactivity using both ELISA and Western blot analysis. However, optimal working dilutions should be determined empirically for each specific application. For Western blot analysis, a dilution range of 1:500 to 1:5000 is recommended, with an initial starting dilution of 1:5000.
Synonyms
DKFZp451O223, SSTNI, TNN1, Troponin I, slow skeletal muscle ,Troponin I, slow-twitch isoform.
Purification Method
TNNI1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT36E7A.
Immunogen
Anti-human TNNI1 mAb, clone PAT36E7A, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TNNI1 protein.
Ig Subclass
Mouse IgG2b heavy chain and Kappa light chain.

Product Science Overview

Troponin I Type 1 (TNNI1)

Troponin I Type 1 is specifically expressed in slow-twitch skeletal muscle fibers. Slow-twitch fibers are responsible for sustained, endurance-type activities and are characterized by their resistance to fatigue. TNNI1 acts as the inhibitory subunit of the troponin complex, blocking actin-myosin interactions and thereby mediating muscle relaxation.

Mouse Anti Human Troponin I Type 1 Antibody

The Mouse Anti Human Troponin I Type 1 antibody is a monoclonal antibody developed in mice that specifically targets the human TNNI1 protein. This antibody is commonly used in research to study muscle physiology, muscle diseases, and the role of TNNI1 in muscle function. It is also utilized in various laboratory techniques, including Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assays (ELISA).

Applications and Importance
  1. Research: The Mouse Anti Human Troponin I Type 1 antibody is widely used in research to investigate the molecular mechanisms underlying muscle contraction and relaxation. It helps in understanding muscle disorders and developing potential therapeutic interventions.
  2. Diagnostics: Although primarily used for research purposes, antibodies against troponin proteins, including TNNI1, have potential diagnostic applications. They can be used to detect muscle damage or diseases characterized by altered troponin levels.
  3. Therapeutics: Understanding the role of TNNI1 in muscle function can lead to the development of targeted therapies for muscle-related diseases. The Mouse Anti Human Troponin I Type 1 antibody can be a valuable tool in this process.
Storage and Handling

The Mouse Anti Human Troponin I Type 1 antibody should be stored at 4°C for short-term use and at -20°C for long-term storage. It is important to avoid repeated freeze-thaw cycles to maintain the antibody’s stability and effectiveness.

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