TNNI2 Human, His

Troponin I Type 2 Human Recombinant, His Tag
Cat. No.
BT16973
Source
E.coli.
Synonyms
AMCD2B, DA2B, FSSV, fsTnI, Troponin I, fast skeletal muscle, Troponin I, fast-twitch isoform.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TNNI2 Human Recombinant produced in E. coli is a single polypeptide chain containing 202 amino acids (1-182) and having a molecular mass of 23.5 kDa. TNNI2 is fused to 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Troponin I, fast skeletal muscle (TNNI2) is a protein belonging to the troponin I gene family. It is a vital component of the troponin complex, which also includes troponin T and troponin C. This complex, together with tropomyosin, plays a crucial role in regulating striated muscle contraction in a calcium-dependent manner. Beyond its role in muscle contraction, TNNI2 has been shown to suppress tumor growth in human ovarian carcinoma. Mutations in the TNNI2 gene are associated with myopathy and distal arthrogryposis type 2B.
Description
Recombinant human TNNI2 protein, expressed in E. coli, is available as a single polypeptide chain. This protein consists of 202 amino acids, comprising residues 1-182 of the native TNNI2 sequence, along with a 20 amino acid His-tag fused at the N-terminus. It has a molecular mass of 23.5 kDa and is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The TNNI2 solution is provided at a concentration of 0.25 mg/mL in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 2 M Urea, 20% glycerol, and 0.2 M NaCl.
Stability
For short-term storage (up to 2-4 weeks), store at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
AMCD2B, DA2B, FSSV, fsTnI, Troponin I, fast skeletal muscle, Troponin I, fast-twitch isoform.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGDEEKRNRA ITARRQHLKS VMLQIAATEL EKEESRREAE KQNYLAEHCP PLHIPGSMSE VQELCKQLHA KIDAAEEEKY DMEVRVQKTS KELEDMNQKL FDLRGKFKRP PLRRVRMSAD AMLKALLGSK HKVCMDLRAN LKQVKKEDTE KERDLRDVGD WRKNIEEKSG MEGRKKMFES ES.

Product Science Overview

Introduction

Troponin I Type 2 is a critical component of the troponin complex, which plays a pivotal role in the regulation of muscle contraction in skeletal and cardiac muscles. The recombinant form of this protein, tagged with a His (histidine) tag, is widely used in research and diagnostic applications due to its ease of purification and high specificity.

Structure and Function

Troponin I is one of the three subunits of the troponin complex, the other two being Troponin T and Troponin C. Troponin I is the inhibitory subunit that binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place, thereby inhibiting muscle contraction. When calcium ions bind to Troponin C, a conformational change occurs, leading to the displacement of Troponin I and allowing muscle contraction to proceed.

Recombinant Production

The recombinant form of Troponin I Type 2 is produced using Escherichia coli (E. coli) expression systems. This method involves inserting the gene encoding Troponin I into E. coli, which then produces the protein. The His tag, typically consisting of six histidine residues, is added to the N-terminus of the protein. This tag facilitates the purification of the protein using immobilized metal ion affinity chromatography (IMAC), which exploits the affinity of histidine residues for metal ions like nickel or cobalt .

Applications

Recombinant Troponin I Type 2 with a His tag is used in various applications, including:

  • Research: It is used to study the molecular mechanisms of muscle contraction and the role of troponin in cardiac and skeletal muscle physiology.
  • Diagnostics: Troponin I is a well-known biomarker for myocardial infarction (heart attack). The recombinant form is used to develop and calibrate diagnostic assays for detecting elevated levels of troponin in the blood, which indicates cardiac muscle damage .
  • Drug Development: It is used in high-throughput screening assays to identify potential therapeutic compounds that can modulate the function of the troponin complex.
Properties
  • Purity: The recombinant protein is typically purified to >90% purity, making it suitable for various biochemical assays .
  • Molecular Weight: The molecular weight of the recombinant Troponin I Type 2 with a His tag is approximately 26.1 kDa .
  • Storage: The protein is usually supplied in a liquid form and should be stored at -20°C or -80°C to maintain stability. It is important to avoid repeated freeze-thaw cycles to prevent degradation .

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