TNNI2 Human

Troponin I Type 2 (Human Recombinant) is a crucial protein used in various clinical and research applications, particularly in the diagnosis of cardiac conditions. This article delves into the background, structure, and significance of this protein.

Troponin I is a part of the troponin complex, which also includes troponin T and troponin C. This complex plays a pivotal role in the regulation of muscle contraction in skeletal and cardiac muscles. Troponin I specifically inhibits the interaction between actin and myosin, thereby regulating muscle contraction .

The recombinant form of Troponin I Type 2 is produced using Escherichia coli (E. coli) expression systems. This method ensures high purity and consistency, making it suitable for various analytical and diagnostic applications . The recombinant protein typically includes the full-length human sequence, ensuring it closely mimics the natural protein found in the human body.

Troponin I is a critical biomarker for diagnosing acute myocardial infarction (AMI) and other cardiac injuries. Elevated levels of troponin I in the blood indicate damage to the heart muscle, making it an essential tool in emergency medicine . The development of high-sensitivity assays for troponin I has further improved the accuracy and early detection of cardiac events .

To address the variability in clinical assays for troponin I, standard reference materials have been developed. These materials ensure traceability to the International System of Units (SI) and help harmonize assay results across different laboratories . The National Institute of Standards and Technology (NIST), in collaboration with the AACC/IFCC Cardiac Troponin I Standardization Committee, has developed certified reference materials to improve the reliability of troponin I measurements .