TNFRSF8 igG-His Human

CD30 Ligand Receptor, IgG-His Tag Human Recombinant
Cat. No.
BT28829
Source
Sf9, Baculovirus cells.
Synonyms
Tumor Necrosis Factor Receptor Superfamily Member 8, Tumor Necrosis Factor Receptor Superfamily, Member 8, Lymphocyte Activation Antigen CD30, CD30L Receptor, Ki-1 Antigen, D1S166E, CD30, Cytokine Receptor CD30, CD30 Antigen, Ki-1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

TNFRSF8 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 613 amino acids (19-379 a.a.) and having a molecular mass of 66.7kDa (Molecular size on SDS-PAGE will appear at approximately 25-100kDa). 
TNFRSF8 is expressed with a 252 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Tumor necrosis factor receptor superfamily member 8 (TNFRSF8) is a receptor for TNFSF8/CD30L. It plays a role in regulating cellular growth and the transformation of activated lymphoblasts. Additionally, TNFRSF8 regulates gene expression by activating NF-kappa-B. As a regulator of apoptosis, TNFRSF8 can induce cell death or proliferation depending on the cell type.
Description
TNFRSF8, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain with a molecular mass of 66.7kDa. It comprises 613 amino acids (19-379 a.a.). On SDS-PAGE, the molecular size appears between 25-100kDa. TNFRSF8 is expressed with a 252 amino acid hIgG-His-tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Colorless, sterile-filtered solution.
Formulation
The TNFRSF8 protein solution (0.5mg/ml) is supplied in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 85.0% as determined by SDS-PAGE.
Synonyms
Tumor Necrosis Factor Receptor Superfamily Member 8, Tumor Necrosis Factor Receptor Superfamily, Member 8, Lymphocyte Activation Antigen CD30, CD30L Receptor, Ki-1 Antigen, D1S166E, CD30, Cytokine Receptor CD30, CD30 Antigen, Ki-1.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADPFPQDRPF EDTCHGNPSH YYDKAVRRCC YRCPMGLFPT QQCPQRPTDC RKQCEPDYYL DEADRCTACV TCSRDDLVEK TPCAWNSSRV CECRPGMFCS TSAVNSCARC FFHSVCPAGM IVKFPGTAQK NTVCEPASPG VSPACASPEN CKEPSSGTIP QAKPTPVSPA TSSASTMPVR GGTRLAQEAA SKLTRAPDSP SSVGRPSSDP GLSPTQPCPE GSGDCRKQCE PDYYLDEAGR CTACVSCSRD DLVEKTPCAW NSSRTCECRP GMICATSATN SCARCVPYPI CAAETVTKPQ DMAEKDTTFE APPLGTQPDC NPTPENGEAP ASTSPTQSLL VDSQASKTLP IPTSAPVALS STGKAAAFES RACSLEPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGKHHH HHH.

Product Science Overview

Introduction

The CD30 Ligand Receptor, also known as CD30/TNFRSF8, is a member of the tumor necrosis factor receptor (TNFR) superfamily. It plays a crucial role in the regulation of immune responses and is involved in various cellular processes, including cell proliferation, differentiation, and apoptosis. The recombinant form of this receptor, tagged with IgG-His, is widely used in research to study its functions and interactions.

Structure and Expression

The CD30 receptor is a type I transmembrane glycoprotein expressed on the surface of activated T cells, B cells, and certain types of lymphoma cells. The recombinant version of this receptor is typically produced in human embryonic kidney (HEK293) cells, ensuring proper folding and post-translational modifications . The IgG-His tag facilitates purification and detection of the protein in various experimental setups.

Function and Significance

CD30 interacts with its ligand, CD30L (also known as CD153 or TNFSF8), to mediate a range of biological effects. This interaction is critical in the context of immune responses, particularly in T cell-dependent immune reactions. CD30 signaling can lead to either cell proliferation or apoptosis, depending on the cellular context and the presence of other signaling molecules .

Applications in Research

The recombinant CD30 Ligand Receptor, tagged with IgG-His, is a valuable tool in immunological research. It is used to study the signaling pathways activated by CD30-CD30L interactions and to investigate the role of CD30 in various diseases, including Hodgkin lymphoma and other hematologic malignancies . The His tag allows for easy purification and detection, making it suitable for use in various assays, including ELISA and Western blotting .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

TNFRSF8 Human
CD30 Ligand Receptor Human Recombinant
Cat. No.
BT28746
Source
Sf9, Baculovirus cells.
TNFRSF8 Mouse
CD30 Ligand Receptor Mouse Recombinant
Cat. No.
BT28913
Source
<p><span lang="EN-US">Sf9, Baculovirus cells</span><span dir="RTL" lang="AR-SA">.</span></p>
TNFSF8 Human
CD30 Ligand Human Recombinant
Cat. No.
BT29337
Source
Escherichia Coli.
TNFSF8 Human, Sf9
CD30 Ligand Human Recombinant, Sf9
Cat. No.
BT29396
Source
Sf9, Baculovirus cells.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.