TNFRSF8 Mouse
Sf9, Baculovirus cells.
Tumor Necrosis Factor Receptor Superfamily, Member 8, Lymphocyte Activation Antigen CD30, CD30L Receptor, Ki-1 Antigen, D1S166E, CD30, Cytokine Receptor CD30, CD30 Antigen, Ki-1
Greater than 90.0% as determined by SDS-PAGE.
Description
TNFRSF8 Mouse Recombinant produced in Baculovirus is a single glycosylated polypeptide chain containing 479 amino acids (19-258 aa) and having a molecular mass of 52.2kDa.
TNFRSF8 is fused to a 239 amino acid hIgG-His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Tumor Necrosis Factor Receptor Superfamily, Member 8, Lymphocyte Activation Antigen CD30, CD30L Receptor, Ki-1 Antigen, D1S166E, CD30, Cytokine Receptor CD30, CD30 Antigen, Ki-1
Sf9, Baculovirus cells.
FPTDRPLKTT CAGDLSHYPG EAARNCCYQC PSGLSPTQPC PRGPAHCRKQ CAPDYYVNED GKCTACVTCL PGLVEKAPCS GNSPRICECQ PGMHCCTPAV NSCARCKLHC SGEEVVKSPG TAKKDTICEL PSSGSGPNCS NPGDRKTLTS HATPQAMPTL ESPANDSARS LLPMRVTNLV QEDATELVKV PESSSSKARE PSPDPGNAEK NMTLELPSPG TLPDISTSEN SKEPASTAST LEPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGKHHHHHH
Product Science Overview
CD30L interacts with its receptor, CD30 (also known as TNFRSF8), which is a cell membrane protein of the TNF receptor superfamily . CD30 is expressed by activated, but not resting, T and B cells . The interaction between CD30L and CD30 plays a crucial role in regulating the proliferation of lymphocytes and may also be involved in human immunodeficiency virus (HIV) replication .
As a regulator of apoptosis, CD30 can induce cell death or proliferation depending on the cell type . It has been shown to limit the proliferative potential of autoreactive CD8 effector T cells, thereby protecting the body against autoimmunity . CD30 expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin’s disease, anaplastic large cell lymphoma, and subsets of Non-Hodgkin’s lymphomas . Additionally, CD30 is linked to leukocytes in patients with chronic inflammatory diseases such as lupus erythematosus, asthma, rheumatoid arthritis, and atopic dermatitis .
Recombinant Mouse CD30 Ligand is produced using a mouse myeloma cell line, NS0-derived mouse CD30 Ligand/TNFSF8 protein . The recombinant protein is typically purified to a high degree, with a purity greater than 95% as determined by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining . The endotoxin level is kept below 0.10 EU per 1 μg of the protein by the LAL method .
The activity of the recombinant mouse CD30 Ligand is measured by its ability to stimulate IL-6 secretion by HDLM human Hodgkin’s lymphoma cells . The effective dose (ED50) for this effect is between 5-30 ng/mL in the presence of 10 µg/mL of a cross-linking antibody .
Recombinant Mouse CD30 Ligand is used in various research applications, including studies on cell signaling, apoptosis, and immune response . It is available in both carrier-free and carrier-containing formulations. The carrier-free version does not contain Bovine Serum Albumin (BSA), which can interfere with certain applications .
For optimal stability and storage, the lyophilized protein should be stored at -20 to -70 °C and reconstituted at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin . Once reconstituted, it should be stored under sterile conditions at 2 to 8 °C for up to one month or at -20 to -70 °C for up to three months .
