TNFSF8 Human, Sf9

CD30 Ligand Human Recombinant, Sf9
Cat. No.
BT29396
Source
Sf9, Baculovirus cells.
Synonyms
Tumor Necrosis Factor Superfamily Member 8, Tumor Necrosis Factor (Ligand) Superfamily, Member 8, CD153 Antigen, CD30 Ligand, CD30LG, CD30-L, CD30L, Tumor Necrosis Factor (Ligand) Superfamily Member 8, Tumor Necrosis Factor Ligand 3A, CD30 Antigen Ligand, TNLG3A, CD153, Tumor necrosis factor ligand superfamily member 8, TNFSF8, CD30 ligand.

Appearance
Sterile filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TNFSF8 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 181 amino acids (63-234a.a.) and having a molecular mass of 20.7kDa (Molecular size on SDS-PAGE will appear at approximately 18-40kDa).
TNFSF8 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
CD30 Ligand (TNFSF8) belongs to the tumor necrosis factor (TNF) ligand family. This cytokine binds to TNFRSF8/CD30, a cell surface antigen found on Hodgkin lymphoma and similar blood cancers. When expressed on B cells, TNFSF8 can hinder the process of Ig class switching. TNFSF8 can either boost or suppress lymphoma cell line growth, depending on the cell line. These diverse effects on CD30+ lymphoma cells suggest TNFSF8 plays a complex role in Hodgkin's and certain non-Hodgkin's lymphomas.
Description
Produced in Sf9 insect cells, TNFSF8 is a single, glycosylated polypeptide chain. It comprises 181 amino acids (residues 63-234), resulting in a molecular weight of 20.7 kDa. On SDS-PAGE, its apparent size ranges from 18 to 40 kDa. This TNFSF8 protein features a C-terminal 6-amino acid His tag and undergoes purification using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The TNFSF8 protein solution is provided at a concentration of 0.5 mg/ml. It is formulated in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. To maintain product integrity, minimize repeated freeze-thaw cycles.
Purity
Purity exceeds 85% as assessed by SDS-PAGE analysis.
Synonyms
Tumor Necrosis Factor Superfamily Member 8, Tumor Necrosis Factor (Ligand) Superfamily, Member 8, CD153 Antigen, CD30 Ligand, CD30LG, CD30-L, CD30L, Tumor Necrosis Factor (Ligand) Superfamily Member 8, Tumor Necrosis Factor Ligand 3A, CD30 Antigen Ligand, TNLG3A, CD153, Tumor necrosis factor ligand superfamily member 8, TNFSF8, CD30 ligand.

Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADPQRTDSIP NSPDNVPLKG GNCSEDLLCI LKRAPFKKSW AYLQVAKHLN KTKLSWNKDG ILHGVRYQDG NLVIQFPGLY FIICQLQFLV QCPNNSVDLK LELLINKHIK KQALVTVCES GMQTKHVYQN LSQFLLDYLQ VNTTISVNVD TFQYIDTSTF PLENVLSIFL YSNSDHHHHH H.

Product Science Overview

Introduction

CD30 Ligand, also known as CD153 or TNFSF8, is a membrane-associated glycoprotein that belongs to the Tumor Necrosis Factor (TNF) superfamily. It is a specific ligand for CD30 (also known as TNFRSF8), which is a member of the TNF receptor superfamily. CD30 was originally described as a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies .

Structure and Expression

CD30 Ligand is a type-II membrane-associated protein, meaning it has an extracellular domain, a transmembrane segment, and a cytoplasmic domain. The extracellular domain of CD30 Ligand interacts with the CD30 receptor on target cells. This interaction is crucial for the biological activities mediated by CD30 .

Biological Activities

The CD30-CD30 Ligand interaction plays a significant role in the immune system, particularly in T-cell responses. CD30 Ligand is expressed on activated T cells, B cells, and macrophages. It has pleiotropic biological activities, meaning it can affect various cell types and biological processes. For instance, it is involved in the regulation of immune responses, cell proliferation, and apoptosis .

Role in Disease

CD30 Ligand and its receptor CD30 are overexpressed in several types of lymphomas, including Hodgkin lymphoma and large cell anaplastic non-Hodgkin’s lymphoma. The interaction between CD30 and CD30 Ligand is thought to contribute to the pathophysiology of these diseases by promoting the survival and proliferation of malignant cells .

Recombinant CD30 Ligand (Human, Sf9)

Recombinant CD30 Ligand (Human, Sf9) is produced using the Sf9 insect cell expression system. This system is commonly used for the production of recombinant proteins because it allows for proper protein folding and post-translational modifications. The recombinant form of CD30 Ligand is used in various research applications, including studies on immune regulation, cancer biology, and the development of therapeutic agents targeting the CD30-CD30 Ligand pathway .

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