TNFR Mouse
Tumor Necrosis Factor Receptor Mouse Recombinant
Cat. No.
BT26637
Source
Escherichia Coli.
Synonyms
Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, TNF-R1, Tumor necrosis factor receptor type I, TNF-RI, TNFR-I, p55, p60, CD120a, Tnfrsf1a, Tnfr-1, Tnfr1, FPF, TNF-R, TNFAR, TNFRI, p55-R, TNFR60, Tnfr-2, TNF-R-I, TNF-R55, TNFRp55, TNF-alphaR1, TNFalpha-R1.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
TNFR Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 191 amino acids and having a molecular mass of 21.1kDa.
The TNFR is purified by proprietary chromatographic techniques.
The TNFR is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Tumor necrosis factor receptor 1 (TNFR1), a member of the TNF-receptor superfamily, binds to TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. Two soluble TNF receptors, sTNFR-I and sTNFR-II, neutralize the biological activities of TNF-alpha and TNF-beta. These soluble receptors are thought to increase due to shedding of the extracellular domains of their membrane-bound counterparts. TNF-a, TNFR1, and TNFR2 play roles in cellular differentiation, while TNFR1 and TNFR2 are involved in cell type-specific renal injury. TNFR1 can signal both cell survival and apoptosis. Apoptosis induced by TNFR1 requires two sequential signaling complexes. Additionally, TNFR1 can activate NF-kappaB, mediate apoptosis, and regulate inflammation. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, leading to ligand-independent and enhanced ligand-dependent TNF signaling. TNFR1 contributes to non-cytocidal TNF effects like the anti-viral state and activation of acid sphingomyelinase. Human TNFR1 possesses a major region that regulates cell surface expression. Amniotic fluid of pregnant women contains high levels of soluble TNF receptors. Germline mutations in the extracellular domains of TNFR1 are associated with the autosomal dominant periodic fever syndrome, with impaired receptor clearance suggested as a potential disease mechanism. Familial hibernian fever (FHF) is caused by defects in the TNFRSF1A gene.
Description
Recombinant Mouse TNFR, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 191 amino acids with a molecular weight of 21.1 kDa. The purification of TNFR is carried out using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The TNFR protein was lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS) at pH 7.4.
Solubility
It is recommended to reconstitute the lyophilized TNFR in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized TNFR, while stable at room temperature for 3 weeks, should be stored desiccated at temperatures below -18°C. After reconstitution, TNFR should be stored at 4°C for 2-7 days. For long-term storage, it is recommended to store at -18°C after adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 97.0% by SDS-PAGE analysis.
Biological Activity
The ED₅₀, determined by the ability to inhibit TNF-α mediated cytotoxicity in L-929 cells, is less than 1.0 µg/ml. This corresponds to a specific activity greater than 1000 IU/mg in the presence of 0.1 ng/mL of recombinant murine TNF-α.
Synonyms
Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, TNF-R1, Tumor necrosis factor receptor type I, TNF-RI, TNFR-I, p55, p60, CD120a, Tnfrsf1a, Tnfr-1, Tnfr1, FPF, TNF-R, TNFAR, TNFRI, p55-R, TNFR60, Tnfr-2, TNF-R-I, TNF-R55, TNFRp55, TNF-alphaR1, TNFalpha-R1.
Source
Escherichia Coli.
Amino Acid Sequence
IHPSGVTGLV PSLGDREKRD SLCPQGKYVH SKNNSICCTK CHKGTYLVSD CPSPGRDTVC RECEKGTFTA SQNYLRQCLS CKTCRKEMSQ VEISPCQADK DTVCGCKENQ FQRYLSETHF QCVDCSPCFN GTVTIPCKET QNTVCNCHAG FFLRESECVP CSHCKKNEEC MKLCLPPPLA NVTNPQDSGT A.
Product Science Overview
Structure and Function
TNFR belongs to the tumor necrosis factor receptor superfamily, which includes several receptors that bind to tumor necrosis factors (TNFs). These receptors are involved in various cellular processes, including cell proliferation, differentiation, and death. The TNFR family is characterized by the presence of cysteine-rich domains in their extracellular regions, which are crucial for ligand binding.
Types of TNFR
There are two main types of TNFRs:
- TNFR1 (p55/p60): This receptor is ubiquitously expressed and can bind both soluble and membrane-bound TNF-α. It is involved in mediating most of the TNF-α-induced cellular responses, including apoptosis and inflammation.
- TNFR2 (p75/p80): This receptor is primarily expressed on immune cells and binds preferentially to membrane-bound TNF-α. It plays a role in immune regulation and tissue regeneration.
Recombinant TNFR in Mice
Recombinant TNFR proteins are produced using genetic engineering techniques, where the TNFR gene is inserted into an expression vector and introduced into host cells (such as bacteria or mammalian cells) to produce the protein. The recombinant TNFR (mouse) is used in various research applications to study its role in immune responses and disease mechanisms.
Applications in Research and Medicine
- Cancer Research: TNFRs are studied for their role in tumor progression and metastasis. For instance, TNFRSF25, a member of the TNFR superfamily, has been shown to promote tumor regression when targeted by agonistic antibodies .
- Inflammatory Diseases: TNFRs are involved in the pathogenesis of several inflammatory diseases. Recombinant TNFRs are used to investigate their potential as therapeutic targets for conditions like rheumatoid arthritis and inflammatory bowel disease .
- Immunotherapy: TNFRs are explored for their potential in immunotherapy. For example, TNFRSF25 agonists have shown promise in enhancing T-cell responses and inhibiting tumor growth .
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