TNFR Human, Sf9
Tumor Necrosis Factor Receptor, sf9 Human Recombinant
Cat. No.
BT26545
Source
Sf9,
Baculovirus cells.
Synonyms
Tumor Necrosis Factor Receptor Superfamily Member 9,
Tumor Necrosis Factor Receptor Superfamily, Member 9, T-Cell Antigen 4-1BB
Homolog, 4-1BB Ligand Receptor, T-Cell Antigen ILA, CD137 Antigen, CDw137,
CD137, ILA, Interleukin-Activated Receptor, Homolog Of Mouse Ly63,Induced By
Lymphocyte Activation (ILA), Homolog Of Mouse 4-1BB, Receptor Protein 4-1BB, T
Cell Antigen ILA, 4-1BB.
Appearance
Sterile Filtered colorless solution.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
TNFR produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 411 amino acids (18-186 a.a.) and
having a molecular mass of 45.3kDa. (Migrates at 40-57kDa on SDS-PAGE under
reducing conditions).
Product Specs
Introduction
TNFR1, a member of the TNF-receptor superfamily, binds to TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. Two soluble TNF receptor types, sTNFR-I and sTNFR-II, neutralize the biological activities of TNF alpha and TNF beta. These soluble receptors' levels appear to rise due to the shedding of the membrane-bound receptors' extracellular domains. TNF-a, TNFR1, and TNFR2 play roles in cellular differentiation. TNFR1 and TNFR2 are involved in cell type-specific renal injury. TNFR1 can signal both cell survival and apoptosis, with TNFR1-induced apoptosis requiring two sequential signaling complexes. This receptor activates NF-kappaB, mediates apoptosis, and regulates inflammation. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, leading to ligand-independent and enhanced ligand-dependent TNF signaling. TNFR1 contributes to inducing non-cytocidal TNF effects, including an anti-viral state and the activation of acid sphingomyelinase. Human TNFR1 possesses a major region that controls cell surface expression. Amniotic fluid of pregnant women contains high levels of soluble TNF receptors. Germline mutations in TNFR1's extracellular domains are linked to the autosomal dominant periodic fever syndrome. Impaired receptor clearance is thought to be a mechanism of this disease. Defects in the TNFRSF1A gene cause familial hibernian fever (FHF).
Description
Produced in Sf9 Baculovirus cells, TNFR is a single, glycosylated polypeptide chain comprising 411 amino acids (specifically, amino acids 18-186). It has a molecular mass of 45.3 kDa and migrates at 40-57 kDa on SDS-PAGE under reducing conditions.
Physical Appearance
The product is a sterile, colorless, and filtered solution.
Formulation
The TNFR protein solution has a concentration of 0.5 mg/ml and contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be stored at 4°C. For longer periods, store frozen at -20°C. For long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles.
Purity
The purity is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
Tumor Necrosis Factor Receptor Superfamily Member 9,
Tumor Necrosis Factor Receptor Superfamily, Member 9, T-Cell Antigen 4-1BB
Homolog, 4-1BB Ligand Receptor, T-Cell Antigen ILA, CD137 Antigen, CDw137,
CD137, ILA, Interleukin-Activated Receptor, Homolog Of Mouse Ly63,Induced By
Lymphocyte Activation (ILA), Homolog Of Mouse 4-1BB, Receptor Protein 4-1BB, T
Cell Antigen ILA, 4-1BB.
Source
Sf9,
Baculovirus cells.
Amino Acid Sequence
ADLFERTRSL
QDPCSNCPAG TFCDNNRNQI CSPCPPNSFS SAGGQRTCDI CRQCKGVFRT RKECSSTSNA ECDCTPGFHC
LGAGCSMCEQ DCKQGQELTK KGCKDCCFGT FNDQKRGICR PWTNCSLDGK SVLVNGTKER DVVCGPSPAD
LSPGASSVTP PAPAREPGHS PQLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV
VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI
EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV
DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H.
Product Science Overview
Introduction
The Tumor Necrosis Factor Receptor (TNFR) is a critical component of the immune system, playing a pivotal role in the regulation of immune responses. The recombinant form of this receptor, produced in Sf9 cells, is utilized extensively in research to understand its function and potential therapeutic applications.
TNFR Superfamily
TNFR in Sf9 Cells
The recombinant TNFR produced in Sf9 cells is a glycosylated polypeptide chain containing 411 amino acids, with a molecular mass of approximately 45.3 kDa . Sf9 cells, derived from the fall armyworm (Spodoptera frugiperda), are commonly used in baculovirus expression systems for producing recombinant proteins. This system allows for high-level expression and proper folding of complex proteins, making it ideal for producing functional TNFR.
Function and Significance
TNFR plays a crucial role in the clonal expansion, survival, and development of T cells . It acts as a receptor for TNFSF9 (also known as 4-1BBL), enhancing CD8+ T-cell survival, cytotoxicity, and mitochondrial activity . This makes TNFR an important target for immunotherapy, particularly in cancer treatment, where boosting the immune response against tumors is essential.
Applications in Research
Recombinant TNFR is used in various research applications, including:
- Studying T-cell responses: Understanding how TNFR signaling affects T-cell proliferation and survival.
- Cancer immunotherapy: Investigating the potential of TNFR agonists to enhance anti-tumor immunity.
- Autoimmune diseases: Exploring the role of TNFR in autoimmune conditions and developing targeted therapies.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
