TNFR (22-211) Human

Tumor Necrosis Factor Receptor (22-211 a.a.) Human Recombinant
Cat. No.
BT26320
Source
Escherichia Coli.
Synonyms
CD120a, FPF, MS5, p55, p55-R, p60, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR1-d2,TNFR55, TNFR60, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor superfamily member 1A.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

TNFR Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 213 amino acids (22-211 a.a) and having a molecular mass of 23.6kDa.
TNFR is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
TNFR1, a member of the TNF-receptor superfamily, binds to TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. Two soluble TNF receptor types, sTNFR-I and sTNFR-II, neutralize the biological activities of TNF alpha and TNF beta. These soluble receptors' levels appear to rise due to the shedding of membrane-bound receptors' extracellular domains. TNF-a, TNFR1, and TNFR2 play roles in cellular differentiation. Additionally, TNFR1 and TNFR2 contribute to cell type-specific renal injury. TNFR1 can signal both cell survival and apoptosis. TNFR1-induced apoptosis necessitates two sequential signaling complexes. Furthermore, TNFR1 can activate NF-kappaB, mediate apoptosis, and regulate inflammation. Oxidative stress is known to promote TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNFR1 contributes to inducing non-cytocidal TNF effects, including antiviral state and acid sphingomyelinase activation. Human TNFR1 possesses a major region that regulates cell surface expression. Amniotic fluid in pregnant women contains high levels of soluble TNF receptors. Germline mutations in TNFR1's extracellular domains are linked to the autosomal dominant periodic fever syndrome. Impaired receptor clearance is considered a potential mechanism of the disease. Familial hibernian fever (FHF) is attributed to defects in the TNFRSF1A gene.
Description
Recombinant Human TNFR, produced in E. coli, is a single, non-glycosylated polypeptide chain containing 213 amino acids (specifically, residues 22-211). It has a molecular mass of 23.6 kDa. The TNFR protein is fused to a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
TNFR protein solution at a concentration of 1 mg/ml. The solution contains 20 mM Tris-HCl (pH 8.0) and 10% glycerol.
Stability
For optimal storage, keep the vial at 4°C if it will be used entirely within 2-4 weeks. For longer periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 85% as assessed by SDS-PAGE.
Synonyms
CD120a, FPF, MS5, p55, p55-R, p60, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR1-d2,TNFR55, TNFR60, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor superfamily member 1A.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSIYPSGVI GLVPHLGDRE KRDSVCPQGK YIHPQNNSIC CTKCHKGTYL YNDCPGPGQD TDCRECESGS FTASENHLRH CLSCSKCRKE MGQVEISSCT VDRDTVCGCR KNQYRHYWSE NLFQCFNCSL CLNGTVHLSC QEKQNTVCTC HAGFFLRENE CVSCSNCKKS LECTKLCLPQ IENVKGTEDS GTT.

Product Science Overview

Introduction

The Tumor Necrosis Factor Receptor (TNFR) is a critical component of the immune system, playing a pivotal role in mediating the effects of the Tumor Necrosis Factor (TNF) family of cytokines. The specific variant, TNFR (22-211 a.a.), is a human recombinant protein that has been extensively studied for its role in various cellular processes, including inflammation, apoptosis, and immune response.

Structure and Production

The TNFR (22-211 a.a.) is a recombinant protein produced in Escherichia coli (E. coli). It is a single, non-glycosylated polypeptide chain consisting of 213 amino acids, with a molecular mass of approximately 23.6 kDa . This protein is fused to a 23 amino acid His-tag at the N-terminus, which facilitates its purification through chromatographic techniques .

Function and Mechanism

TNFR1, the receptor for TNF-alpha (TNFSF2) and lymphotoxin-alpha (TNFSF1), is a member of the TNF-receptor superfamily. It is capable of signaling both cell survival and apoptosis, depending on the context of the cellular environment . TNFR1 can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation . The receptor’s ability to induce apoptosis requires two sequential signaling complexes, highlighting its complex role in cellular processes .

Clinical Significance

High levels of soluble TNF receptors, including TNFR1, are found in the amniotic fluid of pregnant women . Germline mutations in the extracellular domains of TNFR1 are linked to autosomal dominant periodic fever syndrome, a condition characterized by recurrent episodes of fever and inflammation . Additionally, defects in the TNFRSF1A gene, which encodes TNFR1, are associated with familial Hibernian fever (FHF) .

Applications in Research and Medicine

The recombinant TNFR (22-211 a.a.) is widely used in research to study the mechanisms of TNF signaling and its implications in various diseases. It serves as a valuable tool for investigating the role of TNF in inflammation, cancer, and autoimmune disorders. Moreover, understanding the interactions between TNFR1 and its ligands can aid in the development of targeted therapies for conditions involving excessive or dysregulated TNF signaling .

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