TPO Human, Biotin

Thyroid Peroxidase Human Recombinant, Biotinylated
Cat. No.
BT19574
Source
Sf9 insect cells.
Synonyms
Thyroid peroxidase, EC 1.11.1.8, TPO, MSA, TPX.
Appearance

Sterile Filtered clear solution.

Purity

Greater than 95% as determined by SDS-PAGE.

Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Thyroid Peroxidase Human Recombinant produced in SF9 is a Biotinylated, glycosylated, polypeptide chain containing 834 amino acids and having a molecular mass of 93 kDa (excluding glycosylation).

The TPO is expressed with a -6xHis tag at C-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Thyroid Peroxidase (TPO) is a major autoantigen in autoimmune thyroid disease. It was previously known as the 'microsomal antigen'. TPO is an integral membrane glycoprotein found only on the apical plasma membrane of follicular epithelial cells. It consists of two identical subunits, each with a molecular weight of approximately 100 kDa. This hemoprotein is crucial for thyroid hormone biosynthesis, catalyzing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin (TG) to form precursors of the thyroid hormones T4 and T3.
Description
Recombinant Human Thyroid Peroxidase, produced in SF9 cells, is a biotinylated and glycosylated polypeptide chain containing 834 amino acids. It has a molecular weight of 93 kDa, excluding glycosylation. The TPO is expressed with a C-terminal -6xHis tag and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
TPO is supplied in a buffer containing 16mM HEPES (pH 7.6), 160mM NaCl, 0.08mM KI, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For long-term storage, freeze at -20°C. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 95% as determined by SDS-PAGE.
Immunological Functions
This product has the following immunological functions: (1) It binds to IgG-type human autoantibodies. (2) Autoantibodies to TPO recognize conformation-dependent epitopes. (3) It can be used in functional streptavidin-based ELISA tests for analyzing positive and negative samples.
Synonyms
Thyroid peroxidase, EC 1.11.1.8, TPO, MSA, TPX.
Source
Sf9 insect cells.

Product Science Overview

Structure and Production

Human recombinant TPO, particularly the biotinylated form, is produced using recombinant DNA technology. This involves the expression of the TPO gene in suitable host cells, such as Sf9 insect cells . The biotinylation of TPO allows for easy detection and purification using streptavidin-based methods. The recombinant TPO is typically a glycosylated polypeptide chain containing 834 amino acids and has a molecular mass of approximately 93 kDa (excluding glycosylation) .

Biological Properties and Functions

TPO is an integral membrane protein located on the apical membrane of thyroid follicular cells. It is essential for the biosynthesis of thyroid hormones, which are critical for regulating metabolism, growth, and development . The enzyme catalyzes two key reactions:

  1. Iodination of Tyrosyl Residues: TPO facilitates the addition of iodine to tyrosine residues in thyroglobulin.
  2. Coupling of Iodotyrosyl Residues: TPO catalyzes the coupling of iodotyrosyl residues to form the precursors of T4 and T3 .
Immunological Functions

TPO is also known to be an autoantigen in autoimmune thyroid diseases, such as Hashimoto’s thyroiditis and Graves’ disease. Autoantibodies against TPO can interfere with its function, leading to thyroid dysfunction .

Applications

Biotinylated human recombinant TPO is widely used in research and diagnostic applications. It is utilized in enzyme-linked immunosorbent assays (ELISA) to detect and quantify TPO antibodies in serum, which is important for diagnosing autoimmune thyroid diseases .

Storage and Stability

Recombinant TPO should be stored at 4°C if used within 2-4 weeks or frozen at -20°C for longer periods. It is important to avoid multiple freeze-thaw cycles to maintain its stability and activity .

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