TPO Mouse, HEK
HEK293.
Thpo, C-mpl ligand,ML, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, MGDF, Myeloproliferative leukemia virus oncogene ligand, Mgdf, Ml, Mpllg, Tpo, thrombopoietin isoform 1.
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range ≤4ng/ml.
Description
TPO Mouse Recombinant produced in HEK293 cells is a single, non-glycosylated polypeptide chain containing 341 amino acids ( 22-356 a.a) and having a molecular mass of 36.4kDa. TPO is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
Thrombopoietin (TPO), also known as MGDF, is a glycoprotein hormone primarily produced in the liver and kidneys. It plays a crucial role in platelet production by stimulating the production and differentiation of megakaryocytes, specialized bone marrow cells responsible for platelet formation. TPO is essential for megakaryocytopoiesis, the process of megakaryocyte proliferation and maturation, and overall platelet production (thrombopoiesis).
Recombinant Mouse TPO, expressed in HEK293 cells, is a non-glycosylated polypeptide chain consisting of 341 amino acids (22-356 a.a). With a molecular weight of 36.4 kDa, this TPO variant has a 6 amino acid His-tag fused at its C-terminus. Purification is achieved through proprietary chromatographic techniques.
The TPO protein solution has a concentration of 0.5mg/ml and is prepared in Phosphate-Buffered Saline (pH 7.4) with 10% glycerol.
For short-term storage (2-4 weeks), keep refrigerated at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity is determined using a cell proliferation assay with MO7e human megakaryocytic leukemic cells. The ED50 is approximately 4 ng/ml.
Biological activity is assessed by the ability to agglutinate human red blood cells, with an ED50 greater than or equal to 25 µg/ml.
Thpo, C-mpl ligand,ML, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, MGDF, Myeloproliferative leukemia virus oncogene ligand, Mgdf, Ml, Mpllg, Tpo, thrombopoietin isoform 1.
HEK293.
SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKFPNRTSG LLETNFSVTA RTAGPGLLSR LQGFRVKITP GQLNQTSRSP VQISGYLNRT HGPVNGTHGL FAGTSLQTLE ASDISPGAFN KGSLAFNLQG GLPPSPSLAP DGHTPFPPSP ALPTTHGSPP QLHPLFPDPS TTMPNSTAPH PVTMYPHPRN LSQETHHHHH H.
Product Science Overview
Thrombopoietin (TPO) is a crucial cytokine involved in the regulation of platelet production. It primarily influences the proliferation and maturation of megakaryocytes, the bone marrow cells responsible for producing platelets. Recombinant forms of TPO, such as Mouse Recombinant Thrombopoietin expressed in Human Embryonic Kidney (HEK) cells, are widely used in research and therapeutic applications.
Recombinant Mouse Thrombopoietin (HEK) is typically produced in HEK293 cells, a human cell line derived from embryonic kidney cells. This recombinant protein is a single, non-glycosylated polypeptide chain containing 341 amino acids, with a molecular mass of approximately 36.4 kDa . The protein is often purified using proprietary chromatographic techniques to ensure high purity and biological activity.
Thrombopoietin plays a vital role in hematopoiesis, particularly in the production of platelets. It binds to its receptor, c-Mpl, on the surface of megakaryocytes and their progenitors, promoting their proliferation and differentiation . This cytokine is also essential for the maintenance of hematopoietic stem cells (HSCs), ensuring a steady supply of blood cells throughout an organism’s life.
Recombinant Mouse Thrombopoietin (HEK) is used in various research applications, including:
- Cell Proliferation Assays: It is used to study the proliferation of megakaryocytes and other hematopoietic cells.
- Stem Cell Research: TPO is crucial for maintaining and expanding hematopoietic stem cells in vitro.
- Drug Development: It is used in the development and testing of drugs targeting platelet production and related disorders.
Recombinant Mouse Thrombopoietin (HEK) is usually supplied in a lyophilized form to ensure stability during storage and transport. It can be reconstituted in phosphate-buffered saline (PBS) and stored at -80°C for long-term use . Proper storage and handling are essential to maintain the protein’s biological activity and prevent degradation.
