TGFB3 Human, Plant
Transforming Growth Factor-Beta 3 Human Recombinant, Plant
Cat. No.
BT5198
Source
Nicotiana benthamiana.
Synonyms
Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
TGFB3 Human Recombinant produced in plant is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 118 amino acids and having a molecular mass of 27.2kDa.
The TGFB3 is fused to 6xHis tag at N-terminus and purified by standard chromatographic techniques.
The TGFB3 is fused to 6xHis tag at N-terminus and purified by standard chromatographic techniques.
Product Specs
Introduction
The transforming growth factor beta (TGF-β) family of cytokines plays a crucial role in embryonic development by mediating cell-to-cell interactions. Mammals possess three isoforms of TGF-β: TGF-β1, TGF-β2, and TGF-β3. These isoforms share a similar structure, each synthesized as a precursor protein that undergoes cleavage to yield a 112-amino acid polypeptide. This polypeptide remains associated with the latent portion of the molecule.
Description
Recombinant Human TGFB3, produced in plants, is a glycosylated polypeptide with a molecular weight of 27.2 kDa. It forms a disulfide-linked homodimer composed of 118 amino acids. A 6xHis tag is fused to the N-terminus to facilitate purification using standard chromatographic methods.
Physical Appearance
White, lyophilized (freeze-dried) powder in a sterile vial.
Formulation
The product is lyophilized from a solution containing 1 mg/ml TGFB3 in 50 mM Tris-HCl buffer at pH 7.4.
Solubility
To reconstitute the lyophilized TGFB3, it is recommended to dissolve it in sterile 5 mM HCl containing 50 µg/ml BSA to achieve a concentration of 0.05 mg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized TGFB3 is stable for up to 3 weeks at room temperature. However, for long-term storage, it is recommended to store the desiccated product at temperatures below -18°C. After reconstitution, TGFB3 should be stored at 4°C for 2-7 days. For extended storage, add a carrier protein (0.1% HSA or BSA) and store below -18°C. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 95.0% using SDS-PAGE analysis.
Biological Activity
The biological activity of TGFB3 is assessed by its ability to inhibit the proliferation of mink lung epithelial (Mv1Lu) cells in culture. The ED50 value is typically less than or equal to 40 ng/ml, corresponding to a specific activity of 25,000 Units/mg.
Synonyms
Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.
Source
Nicotiana benthamiana.
Amino Acid Sequence
HHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKG
YYANFCSGPCPYLRSADTTHSTVLGLY
NTLNPEASASP
CCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS.
YYANFCSGPCPYLRSADTTHSTVLGLY
NTLNPEASASP
CCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS.
Product Science Overview
Structure and Production
The human recombinant TGF-β3 produced in plants is a disulfide-linked homodimeric, glycosylated polypeptide chain containing 118 amino acids and has a molecular mass of 27.2 kDa . This recombinant protein is fused to a 6xHis tag at the N-terminus and purified using standard chromatographic techniques .
Biological Functions
TGF-β3 plays a crucial role in several biological processes:
- Cell Differentiation and Embryogenesis: It regulates molecules involved in cellular adhesion and extracellular matrix (ECM) formation, which are essential during the development of various tissues .
- Palate Development: TGF-β3 is vital for the fusion of epithelial cells during palate development. Without it, mammals can develop cleft palate .
- Lung Development: It also regulates cell adhesion and ECM formation in lung tissue, playing a significant role in lung development .
- Wound Healing: TGF-β3 orchestrates the movements of epidermal and dermal cells during wound healing .
Clinical Research and Applications
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