TGFB3 (207 a.a.) Human

Transforming Growth Factor-Beta 3 (207 a.a.) Human Recombinant
Cat. No.
BT4853
Source
Escherichia Coli.
Synonyms
Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.
Appearance
Sterile Filtered clear solution.
Purity
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

TGFB3 Human Recombinant protein encoding amino acids 644-850 and total molecular mass of 50 kDa (Including GST Tag).

Product Specs

Introduction
The transforming growth factor beta (TGF-beta) family of cytokines plays a crucial role in embryonic development by mediating cell-to-cell interactions. In mammals, three TGF-beta isoforms (TGF-beta 1, TGF-beta 2, and TGF-beta 3) have been identified. These isoforms share a similar protein structure. Each is synthesized as a larger precursor protein that undergoes cleavage to release a 112-amino acid polypeptide. This active polypeptide remains associated with the remaining portion of the precursor molecule, forming a complex known as the latent TGF-beta complex.
Description
This product consists of the recombinant human TGF-beta 3 protein. It encompasses amino acids 644 to 850 of the full-length protein and has a total molecular weight of 50 kDa, including the GST tag.
Physical Appearance
The product is a clear solution that has been sterilized by filtration.
Formulation
This product is supplied as a 100 μl solution of purified human TGF-beta 3 protein at a concentration of 100 μg/ml. The buffer consists of 50 mM Tris-Acetate (pH 7.5), 1 mM EDTA, and 20% glycerol.
Stability
While the recombinant human TGF-beta 3 protein remains stable at 4°C for up to one week, it is recommended to store it at -20°C for optimal long-term stability. The addition of a carrier protein such as 0.1% HSA or BSA is advisable for extended storage. To maintain protein integrity, avoid repeated freeze-thaw cycles.
Applications
This product is suitable for various applications, including: (a) Enzyme-linked immunosorbent assay (ELISA), (b) Western blotting, (c) Inhibition assays.
Synonyms
Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.
Source
Escherichia Coli.

Product Science Overview

Introduction

Transforming Growth Factor-Beta 3 (TGF-β3) is a member of the TGF-β superfamily, which includes TGF-β1 and TGF-β2. These cytokines play crucial roles in cellular processes such as proliferation, differentiation, and apoptosis. TGF-β3, in particular, is known for its involvement in embryogenesis, tissue regeneration, and immune regulation.

Structure and Composition

The human recombinant TGF-β3 is a 207 amino acid protein. It is typically produced in an E. coli expression system and purified to a high degree of purity, often exceeding 98% as determined by SDS-PAGE and HPLC analyses . The recombinant form is a homodimer, consisting of two identical 112 amino acid subunits linked by a single disulfide bond .

Biological Activity

TGF-β3 is highly conserved across species, with human and mouse TGF-β3 demonstrating 100% sequence homology . This conservation underscores its fundamental role in biological processes. The protein is secreted in a precursor form, which includes a bioactive C-terminal domain and an N-terminal latency-associated peptide (LAP). The mature protein functions as a disulfide-linked homodimer .

Functions and Applications

TGF-β3 exerts pleiotropic effects, including:

  • Suppression of B- and T-cell effector activity: This makes it a critical player in immune regulation.
  • Mediation of tissue healing: TGF-β3 is involved in wound healing and tissue regeneration.
  • Suppression of tumor proliferation: It has been shown to inhibit the growth of certain cancer cells .

Additionally, TGF-β3 promotes the expansion of CD4+CD25+ T-cells, which are essential for maintaining immune tolerance and preventing autoimmunity .

Research and Clinical Implications

The versatility of TGF-β3 extends to various research applications, including:

  • Stem cell differentiation: It is used to guide the differentiation of stem cells into specific cell types.
  • T-cell regulation and differentiation: TGF-β3 is crucial for studying immune responses and developing immunotherapies.
  • Functional assays: It is employed in assays such as ELISA, Western Blotting, and inhibition assays to explore its biological activities .

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