TGFB3 Human, HEK

Transforming Growth Factor-Beta 3 Human Recombinant, HEK
Cat. No.
BT5132
Source
HEK.
Synonyms
Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95% as obsereved by SDS-PAGE.
Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

TGF-b 3 Human Recombinant produced in HEK cells is a non-glycosylated homodimer, having a total molecular weight of 25kDa.
The TGF-b 3 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Transforming growth factor betas (TGF-betas) are signaling molecules that play a crucial role in regulating cell growth and differentiation during embryonic development. In mammals, there are three known isoforms: TGF-beta 1, TGF-beta 2, and TGF-beta 3. All three isoforms share a similar structure, initially synthesized as precursor proteins that undergo cleavage to release a 112-amino acid polypeptide. This active polypeptide remains associated with the latent portion of the molecule.
Description
Recombinant human TGF-beta 3, expressed in HEK cells, is a non-glycosylated homodimer with a molecular weight of 25 kDa. It is purified using proprietary chromatographic techniques, ensuring high purity.
Physical Appearance
White, lyophilized powder, sterile-filtered.
Formulation
The TGF-beta 3 is provided as a lyophilized powder, formulated at a concentration of 1 mg/mL in phosphate-buffered saline (PBS).
Solubility
To reconstitute the lyophilized TGF-beta 3, it is recommended to dissolve it in sterile 4 mM hydrochloric acid (HCl) containing 0.1% endotoxin-free recombinant human serum albumin (HSA).
Stability
Lyophilized TGF-beta 3 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store the desiccated product below -18°C. Once reconstituted, TGF-beta 3 should be stored at 4°C for 2-7 days. For extended storage, it is advisable to store it below -18°C. Adding a carrier protein, such as 0.1% HSA or bovine serum albumin (BSA), can enhance long-term stability. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the protein is greater than 95%, as determined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity of TGF-beta 3 is measured by its ability to inhibit interleukin-4 (IL-4)-induced proliferation of mouse HT-2 cells (BALB/c spleen cells activated by sheep erythrocytes in the presence of IL-2). The specific activity is typically in the range of 0.1-0.5 ng/mL.
Synonyms
Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.
Source
HEK.

Product Science Overview

Introduction

Transforming Growth Factor-Beta 3 (TGF-β3) is a member of the TGF-β superfamily, which consists of multifunctional peptides that regulate various cellular processes. TGF-β3 is particularly significant due to its role in cell growth, differentiation, and development. The recombinant form of TGF-β3, expressed in Human Embryonic Kidney (HEK) 293 cells, is widely used in research and therapeutic applications.

Gene and Protein Structure

The TGF-β3 gene is located on human chromosome 14q24.3 . The protein is synthesized as a precursor that undergoes proteolytic processing to produce a mature, biologically active form. TGF-β3 is a homodimeric protein with a molecular weight of approximately 25 kDa .

Biological Functions

TGF-β3 plays a crucial role in maintaining immune homeostasis by regulating lymphocyte proliferation, apoptosis, hematopoiesis, and embryogenesis . It is involved in various cellular processes, including:

  • Cell Growth and Differentiation: TGF-β3 is a potent inhibitor of cell proliferation and promotes differentiation in various cell types .
  • Chondrogenic Differentiation: It specifically promotes the differentiation of chondrocytes, which are essential for cartilage formation .
  • Wound Healing and Tissue Repair: TGF-β3 is critical for tissue restoration and scarless tissue repair, making it a valuable target for regenerative medicine .
Clinical Significance

TGF-β3 has both tumor-suppressing and tumor-promoting activities, depending on the stage of carcinogenesis. In the early stages, it acts as a tumor suppressor, while in later stages, it promotes tumor progression by inducing epithelial-mesenchymal transition and stimulating angiogenesis . Additionally, mutations in the TGF-β3 gene are associated with non-syndromic cleft palate only (NS CPO), a rare congenital disease .

Recombinant TGF-β3 (HEK)

The recombinant form of TGF-β3 is produced in HEK 293 cells, which ensures proper folding and post-translational modifications. This form is lyophilized from a solution of 50 mM NaOAc, pH 3.7, and is available as a highly pure, endotoxin-tested product suitable for cell culture applications .

Applications in Research and Medicine

Recombinant TGF-β3 is widely used in various research and therapeutic applications, including:

  • Cell Culture: It is used to study cell growth, differentiation, and signaling pathways in vitro .
  • Regenerative Medicine: Due to its role in tissue repair and wound healing, TGF-β3 is explored for therapeutic applications in regenerative medicine .
  • Cancer Research: Its dual role in tumor suppression and promotion makes it a valuable target for cancer research .

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