TARC Macaque
Escherichia Coli.
C-C motif chemokine 17, Small-inducible cytokine A17, Thymus and activation-regulated chemokine, CC chemokine TARC, ABCD-2, CCL17, CCL-17, SCYA17, TARC, A-152E5.3, MGC138271, MGC138273.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Thymus and Activation Regulated Chemokine (CCL17) Rhesus Macaque Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 71 amino acid and having a molecular mass of approximately 8.1kDa.
TRAC is purified by proprietary chromatographic techniques.
Product Specs
The TARC cDNA encodes a precursor protein consisting of 94 amino acids, including a 23 amino acid signal peptide. This signal peptide is cleaved to yield the mature secreted protein, which comprises 71 amino acids. Belonging to the CC chemokine family, CCL-17 shares approximately 24-29% amino acid sequence identity with other chemokines like RANTES, MIP-1a, MIP-1b, MCP-1, MCP-2, MCP-3, and I-309. TARC expression is primarily observed in the thymus, with lower levels detected in the lung, colon, and small intestine. Additionally, transient expression is observed in stimulated peripheral blood mononuclear cells. Recombinant TARC exhibits chemotactic activity towards T cell lines but not monocytes or neutrophils. CCL-17 specifically binds to CCR4, a receptor selectively expressed on T cells. As one of the few Cys-Cys (CC) cytokine genes located on chromosome 16's q arm, CCL17 displays chemotactic activity for T lymphocytes but not monocytes or granulocytes. CCL17 interacts with chemokine receptors CCR4 and CCR8, playing a crucial role in T cell development within the thymus and the trafficking and activation of mature T cells.
Recombinant Thymus and Activation Regulated Chemokine (CCL17) from Rhesus Macaque, produced in E. coli, is a non-glycosylated polypeptide chain consisting of 71 amino acids. It has a molecular weight of approximately 8.1 kDa. The purification of TRAC is achieved using proprietary chromatographic methods.
Sterile Filtered White lyophilized (freeze-dried) powder.
Lyophilized from a 0.2 µm filtered solution in 20mM PB, 0.02% Tween-20 and 300mM NaCl, at a pH of 7.4.
To reconstitute the lyophilized Thymus and Activation Regulated Chemokine Rhesus Macaque, it is recommended to dissolve it in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Lyophilized TRAC remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated below -18°C. Once reconstituted, Thymus and Activation Regulated Chemokine Rhesus Macaque should be stored at 4°C for 2-7 days. For future use, store below -18°C. Avoid repeated freeze-thaw cycles.
The purity is determined to be greater than 97.0% using the following methods:
(a) RP-HPLC analysis.
(b) SDS-PAGE analysis.
C-C motif chemokine 17, Small-inducible cytokine A17, Thymus and activation-regulated chemokine, CC chemokine TARC, ABCD-2, CCL17, CCL-17, SCYA17, TARC, A-152E5.3, MGC138271, MGC138273.
Escherichia Coli.
ARGTNVGREC CLKYFKGAIP LRKLKTWYQT SEDCSRDAIV FVTVQNKAIC SDPNDKKVKK ALKYLQSLER S.
Product Science Overview
Thymus and Activation Regulated Chemokine (TARC), also known as CCL17, is a member of the CC chemokine family. It plays a crucial role in the immune system by attracting T cells to sites of inflammation and infection. The recombinant form of CCL17 from Rhesus Macaque is produced using advanced biotechnological methods, often involving expression in E. coli.
CCL17 is involved in the chemotaxis of T-helper cells, particularly type 2 helper T cells (Th2), cutaneous lymphocyte skin-localizing T cells, and regulatory T cells . It mediates its effects by binding to the CCR4 receptor, which is expressed on these cells . This interaction is crucial for the immune response, as it helps direct T cells to sites where they are needed to combat infections or inflammation.
Elevated levels of CCL17 have been associated with various eosinophilic disorders, including allergic asthma and atopic dermatitis . The chemokine’s role in these conditions highlights its importance in type 2 immune responses. Additionally, CCL17 has been linked to cancer, where it can either aid in immune evasion by attracting regulatory T cells or improve outcomes in certain cancers like melanoma .
Recombinant CCL17 from Rhesus Macaque is used in research to study its role in immune responses and its potential therapeutic applications. By understanding how CCL17 functions, scientists can develop new treatments for diseases involving the immune system, such as autoimmune disorders, allergies, and cancer.
