SYT5 Human

Synaptotagmin V Human Recombinant
Cat. No.
BT6991
Source
Escherichia Coli.
Synonyms
Synaptotagmin V, Synaptotagmin 5, synaptotagmin-5, sytV, SytV.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SYT5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 301 amino acids (109-386aa) and having a molecular mass of 33.6kDa.
SYT5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Synaptotagmin V (SYT5) is a member of the synaptotagmin family, a group of type III membrane proteins. These proteins are characterized by cytoplasmic repeats similar to protein kinase C regulatory (C2) domains, which are known to bind calcium. Synaptotagmins act as negative regulators of vesicle fusion, permitting fusion only in the presence of calcium. They also function as calcium receptors or sensor molecules. SYT5 has been linked to diseases such as labyrinthitis and thyroiditis.
Description
Recombinant human SYT5, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 301 amino acids (109-386aa). It has a molecular mass of 33.6kDa. A 23 amino acid His-tag is fused to the N-terminus of SYT5, and the protein is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
SYT5 protein solution at a concentration of 0.25mg/ml in a buffer containing 20mM Tris-HCl (pH 8.0), 40% glycerol, 0.2M NaCl, and 2mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advised. It is important to avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
Synaptotagmin V, Synaptotagmin 5, synaptotagmin-5, sytV, SytV.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSLGRLQYS LDYDFQSGQL LVGILQAMGL AALDLGGSSD PYVRVYLLPD KRRRYETKVH RQTLNPHFGE TFAFKVPYVE LGGRVLVMAV YDFDRFSRND AIGEVRVPMS SVDLGRPVQA WRELQAAPRE EQEKLGDICF SLRYVPTAGK LTVIVLEAKN LKKMDVGGLS DPYVKVHLLQ GGKKVRKKKT TIKKNTLNPY YNEAFSFEVP CDQVQKVQVE LTVLDYDKLG KNEAIGRVAV GAAAGGAGLR HWADMLANPR RPIAQWHSLR PPDRVRLLPA P

Product Science Overview

Introduction

Synaptotagmin V is a member of the synaptotagmin family, which consists of membrane-trafficking proteins characterized by their role in neurotransmitter release and hormone secretion. These proteins are integral to the process of exocytosis, where neurotransmitters are released from synaptic vesicles into the synaptic cleft .

Structure

Synaptotagmin V, like other synaptotagmins, contains an N-terminal transmembrane region, a variable linker, and two C-terminal C2 domains (C2A and C2B). These C2 domains are crucial for calcium binding, which is essential for the protein’s function as a calcium sensor .

Function

Synaptotagmin V plays a pivotal role in the regulation of neurotransmitter release. It acts as a calcium sensor, detecting the influx of calcium ions (Ca²⁺) into presynaptic nerve terminals. This calcium binding triggers a series of events that lead to the fusion of synaptic vesicles with the presynaptic membrane, thereby releasing neurotransmitters into the synaptic cleft .

Mechanism of Action

The mechanism by which synaptotagmin V facilitates neurotransmitter release involves its interaction with the SNARE complex. The SNARE complex is composed of proteins such as synaptobrevin, syntaxin, and SNAP-25, which drive the fusion of synaptic vesicles with the presynaptic membrane. Synaptotagmin V binds to these SNARE proteins in a calcium-dependent manner, promoting the fusion process .

Recombinant Synaptotagmin V

Recombinant human synaptotagmin V is produced using Escherichia coli expression systems. This recombinant protein is typically purified to a high degree of purity (>90%) and is validated using techniques such as SDS-PAGE and mass spectrometry . Recombinant synaptotagmin V is used in various research applications to study its role in neurotransmitter release and to explore potential therapeutic applications.

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