SYT1 Human

Synaptotagmin I Human Recombinant
Cat. No.
BT6594
Source
Escherichia Coli.
Synonyms
Synaptotagmin-1, Synaptotagmin I, SytI, p65, SYT1, SVP65, SYT.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SYT1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 256 amino acids (136-382 a.a) and having a molecular mass of 29.5kDa.
SYT1 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Synaptotagmin-1 (SYT1), a member of the synaptotagmin family, possesses two C2 domains and plays a crucial role as a calcium sensor in synaptic vesicle trafficking and exocytosis within the brain. This protein is vital for neurotransmitter release and may regulate membrane interactions during synaptic vesicle trafficking at the synapse's active zone. SYT1 exhibits binding affinity for acidic phospholipids, specifically those with an acidic head group and a diacyl backbone. Additionally, it can bind to neurexins, syntaxin, and AP2 in a calcium-independent manner.
Description
Produced in E. coli, this non-glycosylated SYT1 polypeptide comprises amino acids 136-382, resulting in a 29.5 kDa protein. An 8-amino acid His-tag is fused to the C-terminus to facilitate purification using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The SYT1 protein solution (0.25 mg/ml) is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 2 mM DTT, 20% glycerol, and 100 mM NaCl.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. Long-term storage requires freezing at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Repeated freezing and thawing should be avoided.
Purity
The purity of SYT1 is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Synaptotagmin-1, Synaptotagmin I, SytI, p65, SYT1, SVP65, SYT.
Source
Escherichia Coli.
Amino Acid Sequence
MEPKEEEKLG KLQYSLDYDF QNNQLLVGII QAAELPALDM GGTSDPYVKV FLLPDKKKKF ETKVHRKTLN PVFNEQFTFK VPYSELGGKT LVMAVYDFDR FSKHDIIGEF KVPMNTVDFG HVTEEWRDLQ SAEKEEQEKL GDICFSLRYV PTAGKLTVVI LEAKNLKKMD VGGLSDPYVK IHLMQNGKRL KKKKTTIKKN TLNPYYNESF SFEVPFEQIQ KVQVVVTVLD YDKIGKNDAI GKVFVGYNLE HHHHHH.

Product Science Overview

Structure and Function

Synaptotagmin I is characterized by:

  • An N-terminal transmembrane region (TMR)
  • A variable linker
  • Two C-terminal C2 domains (C2A and C2B)

The C2 domains are essential for calcium binding, which is a critical function of synaptotagmin I. When calcium ions bind to these domains, synaptotagmin I acts as a calcium sensor, triggering the release of neurotransmitters by facilitating the fusion of synaptic vesicles with the presynaptic membrane .

Role in Neurotransmitter Release

Synaptotagmin I is integral to the neurotransmitter release cycle. It senses calcium ion concentrations as low as 10 parts per million (ppm) and signals the SNARE complex to open fusion pores, allowing neurotransmitters to be released into the synaptic cleft . This process is vital for synaptic transmission and communication between neurons.

Interactions

Synaptotagmin I interacts with several other proteins, including:

  • SNAP-25
  • Syntaxin 1A (STX1A)
  • S100A13

These interactions are crucial for the proper functioning of synaptotagmin I in neurotransmitter release .

Clinical Significance

Mutations in the SYT1 gene can lead to a rare neurodevelopmental disorder known as SYT1-associated neurodevelopmental disorder or Baker-Gordon Syndrome. This disorder is characterized by intellectual disability, developmental delay, and other neurological symptoms .

Recombinant Synaptotagmin I

Recombinant Synaptotagmin I is produced using recombinant DNA technology, which involves inserting the SYT1 gene into a suitable expression system, such as bacteria or mammalian cells. This allows for the production of large quantities of the protein for research and therapeutic purposes.

Recombinant Synaptotagmin I is used in various studies to understand its role in neurotransmitter release and its potential implications in neurological disorders. It is also used in drug development and screening for compounds that can modulate its activity.

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