STX3 Human
Description
STX3 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Syntaxin-3 is a target membrane protein, also known as a t-SNARE (target-SNAP receptor). SNARE proteins are a large protein family that mediates vesicle fusion, a process that is vital for the transport of molecules within cells. The SNARE complex is formed by the interaction of a vesicle protein (v-SNARE) and a target membrane protein (t-SNARE). Syntaxin-3, along with other syntaxins, plays a pivotal role in this complex formation.
Syntaxin-3 is predominantly localized at the plasma membrane. It cycles between the plasma membrane and the trans-Golgi network, which is a key component of the cell’s sorting and dispatching system for proteins and lipids. This cycling is believed to be important for the regulation of t-SNARE function .
Recombinant human Syntaxin-3 is produced using recombinant DNA technology. This involves inserting the gene encoding Syntaxin-3 into a host organism, such as Escherichia coli (E. coli), which then expresses the protein. The recombinant protein is typically tagged with a polyhistidine tag at the N-terminus to facilitate purification. The recombinant human Syntaxin-3 protein consists of 278 amino acids and has a predicted molecular mass of approximately 32.4 kDa. It is often used in research to study the protein’s function and interactions .
Recombinant Syntaxin-3 is used in various biochemical and biophysical studies to understand its role in membrane fusion and vesicle trafficking. It is also employed in structural studies to elucidate the detailed mechanisms of SNARE complex formation and function. Understanding the function of Syntaxin-3 can provide insights into various cellular processes and may have implications in understanding diseases related to vesicle trafficking and membrane fusion.
Recombinant human Syntaxin-3 is typically provided as a lyophilized powder and should be stored under sterile conditions at -20°C to -80°C. It is recommended to aliquot the protein to avoid repeated freeze-thaw cycles, which can degrade the protein. The protein is stable for up to twelve months when stored properly .
