STX12 Human

Syntaxin-12 is a SNARE (Soluble NSF Attachment Protein Receptor) protein that mediates vesicle fusion at endosomes. The human recombinant form of Syntaxin-12 is typically expressed in Escherichia coli (E. coli) and purified using conventional chromatography techniques . The recombinant protein is often fused to a His-tag at the N-terminus to facilitate purification .

Syntaxin-12 interacts with various proteins to facilitate its role in vesicle transport. One of its key interactions is with the ATP-binding cassette transporter A1 (ABC1), which aids in the cellular release of choline-phospholipids and cholesterol to apolipoprotein A-I (apoA-I) . Additionally, Syntaxin-12 is involved in the biogenesis of platelet α-granules, which are essential for hemostasis and other physiological processes . It interacts physically and functionally with VPS33B and VPS16B, proteins that are crucial for the transport of newly synthesized α-granule proteins through megakaryocyte endosomal compartments .

Recombinant human Syntaxin-12 is primarily used for research purposes. It is not intended for human, diagnostic, or veterinary use . Studies involving Syntaxin-12 have provided insights into its role in vesicle transport and its potential implications in various cellular processes and diseases .

The recombinant protein can be stored at +2°C to +8°C for short-term use (up to one week). For long-term storage, it is recommended to aliquot and store the protein at -20°C to -80°C, avoiding repeated freezing and thawing cycles to maintain its stability .