SPARCL1 Mouse
Sparcl1, Ecm2, hevin, mast9, Sc1, SPARC-like protein 1, Extracellular matrix protein 2, Matrix glycoprotein Sc1.
Greater than 90.0% as determined by SDS-PAGE.
Description
SPARCL1 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 642 amino acids (17-650 a.a) and having a molecular mass of 71.7kDa.
SPARCL1 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
Sparcl1, Ecm2, hevin, mast9, Sc1, SPARC-like protein 1, Extracellular matrix protein 2, Matrix glycoprotein Sc1.
IPTSTRFLSD HSNPTTATLV TPEDATVPIA GVEATADIEN HPSDKAEKPS ALNSEEETHE
QSTEQDKTYS FEVDLKDEED GDGDLSVDPT EGTLTLDLQE GTSEPQQKSL PENGDFPATV
STSYVDPNQR ANITKGKESQ EQPVSDSHQQ PNESSKQTQD LKAEESQTQD PDIPNEEEEE
EEDEEEEEEE EPEDIGAPSD NQEEGKEPLE EQPTSKWEGN REQSDDTLEE SSQPTQISKT
EKHQSEQGNQ GQESDSEAEG EDKAAGSKEH IPHTEQQDQE GKAGLEAIGN QKDTDEKAVS
TEPTDAAVVP RSHGGAGDNG GGDDSKHGAG DDYFIPSQEF LEAERMHSLS YYLKYGGGEE
TTTGESENRR EAADNQEAKK AESSPNAEPS DEGNSREHSA GSCTNFQCKR GHICKTDPQG
KPHCVCQDPE TCPPAKILDQ ACGTDNQTYA SSCHLFATKC RLEGTKKGHQ LQLDYFGACK
SIPACTDFEV AQFPLRMRDW LKNILMQLYE PNPKHGGYLN EKQRSKVKKI YLDEKRLLAG
HPIELLLRD FKKNYHMYVY PVHWQFNELD QHPADRILTH SELAPLRASL VPMEHCITRF
FEECDPNKDK HITLKEWGHC FGIKEEDIDE NLLFLEHHHH HH
Product Science Overview
SPARCL1 is an anti-adhesive protein widely expressed in tissues such as the brain, heart, lung, muscle, and kidney, but notably absent in the liver . The protein contains three conserved structural domains that are implicated in the regulation of cell adhesion, migration, and proliferation . The recombinant mouse SPARCL1 protein is typically produced in cell lines such as NS0 or HEK293 cells and is often tagged with a polyhistidine tag for purification purposes .
SPARCL1 has garnered interest in cancer research due to its down-regulation in various cancers and its potential role as a tumor suppressor . Its ability to regulate angiogenesis, the formation of new blood vessels, makes it a target for therapeutic research in oncology . Additionally, SPARCL1’s role in inhibiting cell growth has been measured in studies using mink lung epithelial cells, where it demonstrated the ability to inhibit cell growth at specific concentrations .
Recombinant mouse SPARCL1 is produced for research purposes and is available in various formulations, including carrier-free versions that do not contain Bovine Serum Albumin (BSA) . This is particularly useful for applications where the presence of BSA could interfere with experimental outcomes . The protein is typically lyophilized and can be reconstituted in sterile PBS for use in various assays .
