LUM Human
Lumican Human Recombinant
Cat. No.
BT21480
Source
Escherichia Coli.
Synonyms
Lumican, LDC, Lumican Proteoglycan, Keratan Sulfate Proteoglycan Lumican, SLRR2D, KSPG Lumican.
Appearance
Sterile Filtered clear solution.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
LUM Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 343 amino acids (19-338a.a) and having a molecular mass of 39kDa.
LUM is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Lumican, also known as LUM, is a member of the small leucine-rich proteoglycan (SLRP) family, which includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. These bifunctional molecules exhibit a unique characteristic: their protein component binds to collagen fibrils, while their highly charged hydrophilic glycosaminoglycans play a role in regulating the spaces between these fibrils. As the primary keratan sulfate proteoglycan in the cornea, lumican is crucial for corneal transparency. Additionally, LUM is found in interstitial collagenous matrices throughout the body, contributing to collagen fibril organization, circumferential growth, epithelial cell migration, and tissue repair. Notably, LUM has been implicated in diseases such as posterior amorphous corneal dystrophy.
Description
Recombinant Human LUM, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 343 amino acids (19-338a.a) with a molecular weight of 39kDa. It features a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
LUM protein solution at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.4M Urea, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. To enhance stability during long-term storage, consider adding a carrier protein such as HSA or BSA (0.1%). Repeated freeze-thaw cycles should be avoided.
Purity
Purity exceeds 90.0% as determined by SDS-PAGE analysis.
Synonyms
Lumican, LDC, Lumican Proteoglycan, Keratan Sulfate Proteoglycan Lumican, SLRR2D, KSPG Lumican.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSQYYDYDF PLSIYGQSSP NCAPECNCPE SYPSAMYCDE LKLKSVPMVP PGIKYLYLRN NQIDHIDEKA FENVTDLQWL ILDHNLLENS KIKGRVFSKL KQLKKLHINH NNLTESVGPL PKSLEDLQLT HNKITKLGSF EGLVNLTFIH LQHNRLKEDA VSAAFKGLKS LEYLDLSFNQ IARLPSGLPV SLLTLYLDNN KISNIPDEYF KRFNALQYLR LSHNELADSG IPGNSFNVSS LVELDLSYNK LKNIPTVNEN LENYYLEVNQ LEKFDIKSFC KILGPLSYSK IKHLRLDGNR ISETSLPPDM YECLRVANEV TLN.
Product Science Overview
Structure and Function
Human Lumican is synthesized as a 338 amino acid precursor that includes an 18 amino acid signal sequence and a 320 amino acid mature chain . The protein is characterized by its leucine-rich repeats, which are essential for its interaction with collagen fibrils and other extracellular matrix components . Lumican is a major component of the cornea, dermal, and muscle connective tissues . It binds to collagen fibrils and regulates interfibrillar spacing through its highly charged hydrophilic glycosaminoglycans .
Biological Role
Lumican is involved in various biological processes, including:
- Collagen Fibril Organization: Lumican regulates the assembly and organization of collagen fibrils, which is essential for maintaining the structural integrity of tissues .
- Corneal Transparency: In the cornea, Lumican plays a critical role in maintaining transparency by regulating collagen fibril spacing .
- Epithelial Cell Migration and Tissue Repair: Lumican modulates cell behavior such as adhesion and migration, contributing to wound healing and tissue repair .
- Immune Response: Lumican interacts with cell surface receptors to regulate cell proliferation, differentiation, inflammation, and the innate and humoral immune systems .
Recombinant Human Lumican
Applications
Recombinant Human Lumican is used in research to:
- Investigate its role in collagen fibril organization and tissue repair.
- Study its interactions with cell surface receptors and its regulatory effects on cell behavior.
- Explore its potential therapeutic applications in wound healing and tissue engineering.
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