LUM Mouse

Lumican Mouse Recombinant
Cat. No.
BT21666
Source
Sf9, Baculovirus cells.
Synonyms

Lum, Ldc, SLRR2D, Lumican, Keratan sulfate proteoglycan lumican, KSPG lumican.

Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

LUM Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 328 amino acids (19-338 a.a) and having a molecular mass of 37.5kDa (Migrates at 40-57kDa on SDS-PAGE under reducing conditions).
LUM is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Lumican, also known as LUM, belongs to the small leucine-rich proteoglycan (SLRP) family, which includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. These bifunctional molecules exhibit a unique characteristic: their protein component binds to collagen fibrils, while their highly charged hydrophilic glycosaminoglycans play a crucial role in regulating the spacing between these fibrils. As the primary keratan sulfate proteoglycan found in the cornea, LUM is also present in interstitial collagenous matrices throughout the body. Its functions encompass regulating collagen fibril organization and growth, maintaining corneal transparency, facilitating epithelial cell migration, and contributing to tissue repair.
Description
Recombinant LUM Mouse, expressed in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It comprises 328 amino acids (19-338 a.a), resulting in a molecular mass of 37.5 kDa. Under reducing conditions on SDS-PAGE, it migrates between 40-57 kDa. The protein is engineered with an 8 amino acid His-tag at its C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
LUM protein solution at a concentration of 0.5mg/ml in Phosphate Buffered Saline (pH 7.4) with 20% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Synonyms

Lum, Ldc, SLRR2D, Lumican, Keratan sulfate proteoglycan lumican, KSPG lumican.

Source
Sf9, Baculovirus cells.
Amino Acid Sequence

QYYDYDIPLF MYGQISPNCA PECNCPHSYP TAMYCDDLKL KSVPMVPPGI KYLYLRNNQI
DHIDEKAFEN VTDLQWLILD LLENSKIK GKVFSKLKQL KKLHINYNNL TESVGPLPKS
LQDLQLTNNK ISKLGSFDGL VNLTFIYLQH NQLKEDAVSA SLKGLKSLEY LDLSFNQMSK
LPAGLPTSLL TLYLDNNKIS NIPDEYFKRF TGLQYLRLSH NELADSGVPG NSFNISSLLE
LDLSYNKLKS IPTVNENLEN YYLEVNELEK FDVKSFCKIL GPLSYSKIKH LRLDGNPLTQ SSLPPDMYEC LRVANEITVN VEHHHHHH.

Product Science Overview

Structure and Synthesis

Mouse Lumican is synthesized as a 338 amino acid precursor, which includes an 18 amino acid signal sequence and a 320 amino acid mature chain . The recombinant form of Lumican is typically produced in mouse myeloma cell lines (NS0-derived) and includes a C-terminal 6-His tag for purification purposes .

Functions

Lumican is involved in several biological processes, including:

  • Collagen Fibrillogenesis: Lumican modulates the formation and organization of collagen fibers, which are critical for the structural integrity of connective tissues .
  • Cell Adhesion: It supports the adhesion of bovine corneal endothelial cells, indicating its role in cellular interactions and tissue maintenance .
  • Tissue Repair and Regeneration: Lumican is implicated in wound healing and tissue repair processes due to its interaction with collagen and other extracellular matrix components .
Applications

Recombinant Mouse Lumican is used in various research applications, including:

  • Cell Culture: It is used to study cell adhesion and migration in vitro .
  • Tissue Engineering: Lumican’s role in collagen organization makes it a valuable tool in tissue engineering and regenerative medicine .
  • Cancer Research: Elevated levels of Lumican have been observed in certain types of cancer, making it a potential biomarker for tumor progression and a target for therapeutic interventions .
Storage and Stability

Recombinant Mouse Lumican is typically lyophilized from a 0.2 μm filtered solution in PBS and should be reconstituted at 200 μg/mL in PBS . It is stable for 12 months when stored at -20 to -70 °C and for 1 month at 2 to 8 °C under sterile conditions after reconstitution .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

LUM Human
Lumican Human Recombinant
Cat. No.
BT21480
Source
Escherichia Coli.
LUM Human, sf9
Lumican Human Recombinant, Sf9
Cat. No.
BT21587
Source
Sf9, Baculovirus cells.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.