LUM Human, sf9
Description
LUM is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Lumican (Human Recombinant, Sf9) is produced in Sf9 Baculovirus cells. It is a single, glycosylated polypeptide chain containing 329 amino acids (19-338 aa) and has a molecular mass of 37.7 kDa . The recombinant protein is expressed with a 6 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques .
Lumican is the main keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body . It regulates collagen fibril organization and circumferential growth, which is essential for maintaining the structural integrity of various tissues . Additionally, lumican plays a role in:
- Corneal Transparency: Lumican is critical for maintaining the transparency of the cornea, which is essential for vision .
- Epithelial Cell Migration: It aids in the migration of epithelial cells, which is important for wound healing and tissue repair .
- Tissue Repair: Lumican is involved in the repair of tissues by regulating collagen fibril organization and promoting cell migration .
Lumican has been implicated in various diseases and conditions. For instance, it is associated with posterior amorphous corneal dystrophy, a condition that affects the cornea . Recent research has also shown that lumican levels are elevated in the lungs of patients with acute respiratory distress syndrome (ARDS) and are linked to disease severity and inflammatory fibrotic processes . Lumican triggers the transdifferentiation of lung fibroblasts into myofibroblasts and epithelial-mesenchymal transition in small airway epithelial cells, possibly via the ERK/Slug pathway .
The recombinant lumican protein is provided as a sterile filtered colorless solution. It is formulated in phosphate-buffered saline (pH 7.4) with 10% glycerol . For short-term storage, it can be kept at 4°C for 2-4 weeks. For long-term storage, it is recommended to store the protein at -20°C with the addition of a carrier protein (0.1% HSA or BSA) to avoid multiple freeze-thaw cycles .
