SPARC Mouse
Sf9, Baculovirus cells.
Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic and Rich in Cysteine
Greater than 95.0% as determined by SDS-PAGE.
Description
SPARC Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 291 amino acids (18-302a.a) and having a molecular mass of 33.3kDa.
SPARC is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
Secreted Protein Acidic and Rich in Cysteine (SPARC), also known as osteonectin, is encoded by the SPARC gene in humans. This glycoprotein plays a crucial role in bone mineralization by binding to calcium. SPARC is synthesized by various cells, including fibroblasts, capillary endothelial cells, platelets, and macrophages, primarily in tissues undergoing morphogenesis and remodeling. Besides calcium, SPARC also exhibits binding affinity for collagen.
Recombinant Mouse SPARC protein, expressed in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain comprising 291 amino acids (18-302a.a) with a molecular weight of 33.3kDa. This protein includes a 6 amino acid His-tag fused at the C-terminus and is purified using proprietary chromatographic techniques.
The SPARC solution is provided at a concentration of 0.25mg/ml and is formulated in Phosphate-Buffered Saline (pH 7.4) with 10% glycerol.
For short-term storage (2-4 weeks), the SPARC solution should be stored at 4°C. For extended storage, it is recommended to store the solution frozen at -20°C. To ensure stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advisable. It is important to avoid repeated freeze-thaw cycles to maintain protein integrity.
The purity of SPARC Mouse Recombinant is determined to be greater than 95.0% as assessed by SDS-PAGE analysis.
Osteonectin, ON, Basement-membrane protein 40, BM-40, SPARC, Secreted Protein acidic and Rich in Cysteine
Sf9, Baculovirus cells.
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Product Science Overview
SPARC is a 40 kDa acidic and cysteine-rich glycoprotein consisting of a single polypeptide chain. It can be divided into four domains:
SPARC is involved in several biological processes, including:
- Bone Mineralization: It binds to calcium and collagen, promoting the formation of mineral crystals in bones .
- ECM Remodeling: Acts as a matricellular chaperone, mediating the disassembly and degradation of ECM networks .
- Cell-Matrix Interactions: Modulates interactions between cells and the ECM, influencing cell adhesion, migration, and proliferation .
- Tumor Biology: Overexpression of SPARC has been linked to various cancers, including breast, prostate, colon, and pancreatic cancers. It promotes angiogenesis, proliferation, and migration of tumor cells .
The mouse recombinant version of SPARC is produced using recombinant DNA technology, where the SPARC gene from mice is inserted into a suitable expression system, such as bacteria or yeast. This allows for the production of large quantities of SPARC protein for research and therapeutic purposes.
