SNCG Human
Greater than 90.0% as determined by SDS-PAGE.
Description
The protein coding region of g-synuclein was amplified by RT-PCR and cloned into an E.coli expression vector. g-synuclein was overexpressed in E. coli and purified to apparent homogeneity by taking advantage of the thermosolubility of the protein and by using conventional column chromatography techniques.
Product Specs
Product Science Overview
Gamma-Synuclein is a presynaptic protein that plays a crucial role in the peripheral nervous system and retina . It is involved in maintaining the integrity of the neurofilament network and modulating axonal architecture during development and in adulthood . The protein consists of 127 amino acids and has a molecular mass of approximately 13,331 Da .
Gamma-Synuclein is implicated in several neurodegenerative diseases, including Parkinson’s Disease (PD) and other synucleinopathies . It is a major component of pathological aggregates found in these disorders. The protein’s role in disease pathogenesis is linked to its ability to form insoluble fibrils that contribute to neuronal dysfunction and cell death .
Recombinant human gamma-synuclein is produced using Escherichia coli (E. coli) expression systems. The recombinant protein is purified to ensure high purity and quality, making it suitable for various research applications, including aggregation studies, seeding experiments, and molecular standards . The protein is typically provided in a lyophilized form and can be reconstituted in buffer solutions for experimental use .
Recombinant gamma-synuclein is widely used in research to study its role in neurodegenerative diseases. It is utilized in experiments to understand the mechanisms of protein aggregation, the formation of pathological inclusions, and the development of potential therapeutic strategies . Additionally, gamma-synuclein is used as a molecular standard in various biochemical assays .
