SNCA (1-140) Antibody
Description
Product Specs
SNCA antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Product Science Overview
Alpha-synuclein is a protein predominantly expressed in the central nervous system, particularly in the neocortex, hippocampus, substantia nigra, thalamus, and cerebellum. It is primarily a neuronal protein but can also be found in neuroglial cells . The human alpha-synuclein protein consists of 140 amino acids and is encoded by the SNCA gene .
Alpha-synuclein is an intrinsically disordered protein, meaning it lacks a stable secondary or tertiary structure in solution. However, it can adopt partial alpha-helical and beta-sheet structures, as well as mostly structured tetrameric states . The protein is divided into three distinct domains:
- N-terminal region (1-60 a.a.): This region is amphipathic and dominated by four 11-residue repeats, including the consensus sequence KTKEGV, which has a structural alpha helix propensity similar to apolipoproteins-binding domains .
- Central hydrophobic region (61-95 a.a.): This region includes the non-amyloid-β component (NAC) region, which is involved in protein aggregation .
- C-terminal region (96-140 a.a.): This region is highly acidic and proline-rich .
Alpha-synuclein is involved in the regulation of dopamine release and transport and may function to induce fibrillization of microtubule-associated protein tau . It also acts as a molecular chaperone in the formation of SNARE complexes .
The mouse anti-human alpha-synuclein antibody is a monoclonal antibody that specifically targets the alpha-synuclein protein. It is used in various research applications, including immunohistochemistry and Western blotting . This antibody is produced by immunizing mice with a synthetic peptide corresponding to the carboxy-terminal sequence of human alpha-synuclein .
The mouse anti-human alpha-synuclein antibody is widely used in research to study the role of alpha-synuclein in neurodegenerative diseases, particularly Parkinson’s disease. It is also used to investigate the protein’s interactions with other cellular components and its involvement in synaptic transmission and membrane trafficking .
