SARS Nucleocapsid PT3851
Greater than 90% as determined by SDS-PAGE gel.
Description
SARS Nucleoprotein antibody clone PT3851 is specific for the nucleocapsid SARS-CoV protein and recognizes the nucleoprotein of SARS-CoV-2 by ELISA.
Product Specs
Protein A affinity purified.
PT3851
Recombinant protein fragment 1-49 a.a. of the SARS nucleoprotein.
Mouse IgG2b.
Product Science Overview
The Mouse Anti SARS Nucleocapsid Clone PT3851 is a monoclonal antibody specifically designed to target the nucleocapsid protein of the Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV). This antibody is widely used in research to detect and study the nucleocapsid protein, which plays a crucial role in the viral life cycle.
The nucleocapsid protein, also known as the nucleoprotein or Protein N, is one of the four main structural proteins of coronaviruses, including SARS-CoV and SARS-CoV-2 . It is the most abundant viral protein in infected cells and is essential for viral RNA binding and packaging into the helical nucleocapsid . The nucleocapsid protein also enhances viral transcription and assembly, making it a multifunctional protein critical for the virus’s replication and propagation .
Clone PT3851 is a monoclonal antibody produced by immunizing mice with a recombinant fragment of the SARS nucleocapsid protein . This clone is highly specific for the nucleocapsid protein and does not cross-react with other coronavirus proteins . The antibody is of the IgG2b isotype and is purified using affinity chromatography on Protein A .
The Mouse Anti SARS Nucleocapsid Clone PT3851 is used in various applications, including:
- Western Blotting: To detect the presence of the nucleocapsid protein in viral samples.
- ELISA: For quantifying the nucleocapsid protein in different samples.
- Immunohistochemistry: To visualize the distribution of the nucleocapsid protein in tissue sections.
- Immunofluorescence: For studying the localization of the nucleocapsid protein within cells .
The nucleocapsid protein is highly immunogenic, and high concentrations of anti-nucleoprotein IgG antibodies have been detected in patients with SARS . This makes the nucleocapsid protein a target of interest for developing diagnostic tools and vaccines against COVID-19 . The Mouse Anti SARS Nucleocapsid Clone PT3851 is instrumental in these research efforts, providing a reliable tool for detecting and studying the nucleocapsid protein.
The antibody is typically supplied in a phosphate-buffered saline solution with 0.09% sodium azide as a preservative . It should be stored at -20°C to maintain its stability and activity . The product is guaranteed for 12 months from the date of dispatch when stored under the recommended conditions .
