SARS Nucleocapsid Monoclonal
Description
Product Specs
SARS Antibody was developed by immunizing mice with a protein fragment amino acids 1-49 from the human SARS Nucleocapsid coronavirus (Genbank accession no. NP_828858). SARS-Nucleocapsid, Monoclonal Antibody is protein-G purified.
Product Science Overview
The SARS-CoV-2 nucleocapsid (N) protein is a crucial structural component of the virus responsible for COVID-19. This protein plays a significant role in the viral life cycle, including replication, transcription, and genome packaging . The N protein is located inside the viral particle, where it associates with the viral RNA to form the ribonucleoprotein core .
The N protein is composed of two main protein domains connected by an intrinsically disordered region (IDR) known as the linker region, with additional disordered segments at each terminus . The N-terminal and C-terminal domains are capable of binding RNA, and the C-terminal domain forms a dimer that is likely to be the native functional state . The N protein is highly immunogenic, meaning it can elicit a strong immune response, making it a target for diagnostic and therapeutic applications .
Monoclonal antibodies (mAbs) are antibodies produced from a single cell lineage made by cloning a unique white blood cell . These antibodies have monovalent affinity, meaning they bind to the same epitope, the part of an antigen recognized by the antibody . Monoclonal antibodies can be engineered to bind to almost any suitable substance, making them valuable tools in biochemistry, molecular biology, and medicine .
Monoclonal antibodies are used in the diagnosis and treatment of various diseases, including cancer, autoimmune disorders, and infectious diseases like COVID-19 . They work by mimicking the action of naturally occurring antibodies, stimulating the immune system to act against disease-causing agents . There are several types of monoclonal antibodies, classified based on the amount of mouse and human antibodies they contain :
- Murine monoclonal antibodies: 100% mouse antibodies.
- Chimeric monoclonal antibodies: More than 65% human antibodies, made by fusing mouse antibodies with human antibodies.
- Humanized monoclonal antibodies: More than 90% human antibodies, made by grafting parts of mouse antibodies onto human antibodies.
- Human monoclonal antibodies: 100% human antibodies, made by inserting the genetic material of mouse antibodies into human antibodies.
A SARS-Nucleocapsid monoclonal antibody is a type of monoclonal antibody specifically designed to target the N protein of the SARS-CoV-2 virus. These antibodies can be used for diagnostic purposes, such as detecting the presence of the virus in a patient’s sample, or for therapeutic purposes, such as neutralizing the virus and preventing it from infecting cells .
The development of SARS-Nucleocapsid monoclonal antibodies involves exposing an animal, such as a mouse, to the N protein of the virus. The animal’s B cells are then harvested, manipulated, and cloned to produce antibodies specific to the N protein . These antibodies can be further engineered to enhance their efficacy and reduce the risk of immune reactions in humans .
