SARS Nucleocapsid (1-49)
Description
The E.Coli derived 32 kDa recombinant protein contains the Nucleocapsid core protein 1-49 amino acids immunodominant regions. SARS Necleocapsid protein 1-49 a.a. is fused to a GST tag.
Product Specs
Product Science Overview
The SARS-Associated Coronavirus Nucleocapsid Core Recombinant, 1-49 amino acids (a.a.), is a segment of the nucleocapsid (N) protein from the SARS-CoV-2 virus. This protein plays a crucial role in the virus’s life cycle, including the packaging of the viral RNA genome and the assembly of virus particles. The N protein is highly immunogenic, making it a significant target for diagnostic and therapeutic applications.
The N protein of SARS-CoV-2 is composed of two major domains: the N-terminal domain (NTD) and the C-terminal domain (CTD), flanked by intrinsically disordered regions. The 1-49 a.a. segment is part of the NTD, which is responsible for binding to the viral RNA genome. This binding is essential for the formation of the ribonucleoprotein complex, which is critical for the virus’s replication and assembly.
The N protein is involved in several key processes in the SARS-CoV-2 life cycle:
- RNA Binding: The NTD binds to the viral RNA, facilitating the formation of the ribonucleoprotein complex.
- Viral Assembly: The N protein interacts with other viral proteins and the host cell machinery to assemble new virions.
- Immune Evasion: The N protein helps the virus evade the host immune response by interfering with various immune signaling pathways.
The recombinant Nucleocapsid Core, 1-49 a.a., is produced using recombinant DNA technology. This involves cloning the gene segment encoding the 1-49 a.a. of the N protein into an expression vector, which is then introduced into a host cell (such as E. coli) for protein production. The recombinant protein is subsequently purified for use in research and diagnostic applications.
The recombinant Nucleocapsid Core, 1-49 a.a., has several important applications:
- Diagnostic Assays: Due to its high immunogenicity, the N protein is used in serological assays to detect antibodies against SARS-CoV-2 in patient samples.
- Vaccine Development: The N protein is being explored as a potential target for vaccine development, as it induces a strong immune response.
- Research: The recombinant N protein is used in various research studies to understand the structure, function, and interactions of the N protein, as well as its role in the viral life cycle.
