SARS Core (340-390)
Escherichia Coli.
Sterile Filtered clear solution.
SARS Core protein is >95% pure as determined by 10% PAGE (coomassie staining).
Description
The E.Coli derived 32kDa recombinant protein contains the Nucleocapsid core protein 340-390 amino acids immunodominant regions.
Product Specs
Escherichia Coli.
SARS Core protein was purified by proprietary chromatographic technique.
SARS Core protein is Immunoreactive with sera of SARS-infected individuals.
Product Science Overview
The SARS-Associated Coronavirus (SARS-CoV) nucleocapsid (N) protein is a critical structural component of the virus. It plays a pivotal role in the viral life cycle, including RNA genome packaging, replication, and transcription. The nucleocapsid protein is highly immunogenic and has been a target for diagnostic and therapeutic interventions.
The nucleocapsid protein of SARS-CoV is composed of multiple domains, each contributing to its function. The region spanning amino acids 340-390 is part of the C-terminal domain, which is involved in RNA binding and oligomerization. This region is crucial for the formation of the ribonucleoprotein complex, which is essential for the encapsidation of the viral RNA genome .
The recombinant nucleocapsid core (340-390 a.a) is a synthesized fragment of the N protein, often used in research and diagnostic applications. This recombinant protein is produced using various expression systems, such as bacterial or mammalian cells, to ensure high yield and purity. The recombinant fragment retains the functional properties of the native protein, making it a valuable tool for studying the virus’s biology and developing diagnostic assays .
- Diagnostic Development: The recombinant nucleocapsid core is used in serological assays to detect antibodies against SARS-CoV. Its high immunogenicity makes it an ideal candidate for developing sensitive and specific diagnostic tests .
- Vaccine Research: The nucleocapsid protein is a potential target for vaccine development. Studies have shown that the N protein can elicit strong immune responses, making it a promising candidate for inclusion in vaccine formulations .
- Therapeutic Research: Understanding the structure and function of the nucleocapsid protein can aid in the development of antiviral drugs. Inhibitors targeting the N protein’s RNA-binding domain could potentially disrupt the viral life cycle .
