SAA1 Monkey
Serum Amyloid A (APO-SAA1) Rhesus Macaque Recombinant
Cat. No.
BT22211
Source
Escherichia Coli.
Synonyms
Serum amyloid A protein, SAA, Amyloid protein A, Amyloid fibril protein AA, SAA1, SAA2, PIG4, TP53I4, MGC111216.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
SAA1 monkey Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 104 amino acids and having a total molecular mass of 11.8 kDa.
SAA1 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Serum amyloid A1 (SAA1) is an acute-phase protein primarily produced by the liver in response to inflammation. Its levels can rise dramatically during inflammatory events. SAA1 is believed to play a role in immune modulation, including tissue repair, antimicrobial activity, and directing immune cells to infection sites. However, persistently elevated SAA1 levels are associated with the development of secondary amyloidosis, a condition characterized by the accumulation of amyloid fibrils in various tissues and organs. This accumulation, particularly of the SAA1 cleavage product amyloid protein A, can lead to organ damage. Secondary amyloidosis is often linked to chronic inflammatory diseases like rheumatoid arthritis.
Description
This product consists of recombinant SAA1 protein derived from monkey and produced in E. coli. It is a single, non-glycosylated polypeptide chain with 104 amino acids, resulting in a molecular mass of 11.8 kDa. The protein undergoes purification using proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The protein was lyophilized with 1x PBS at a pH of 7.4.
Solubility
To reconstitute the lyophilized SAA1, it is recommended to dissolve it in sterile 18 megaohm-cm H2O at a concentration of at least 100 micrograms/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized SAA1 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it should be stored desiccated at a temperature below -18 degrees Celsius. After reconstitution, SAA1 can be stored at 4 degrees Celsius for 2-7 days. For extended storage, it is advised to store it at a temperature below -18 degrees Celsius. The addition of a carrier protein like HSA or BSA at a concentration of 0.1% is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of this product is greater than 97.0%, as determined by two methods: Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) and SDS-PAGE analysis.
Biological Activity
The biological activity of this product is assessed based on its ability to chemoattract human monocytes. This chemoattractant activity is evaluated within a concentration range of 1.0 to 10.0 ng/ml.
Synonyms
Serum amyloid A protein, SAA, Amyloid protein A, Amyloid fibril protein AA, SAA1, SAA2, PIG4, TP53I4, MGC111216.
Source
Escherichia Coli.
Amino Acid Sequence
RSWFSFLGEA YDGARDMWRA YSDMKEANYK NSDKYFHARG NYDAAQRGPG GVWAAEVISD ARENIQKLLG RGAEDTLADQ AANEWGRSGK DPNHFRPAGL PEKY.
Product Science Overview
Expression and Production
In the context of recombinant protein production, Serum Amyloid A (APO-SAA1) Rhesus Macaque Recombinant is typically produced using E. coli expression systems. The recombinant protein is a single, non-glycosylated polypeptide chain containing 104 amino acids and has a molecular mass of approximately 11.8 kDa . The protein is purified using proprietary chromatographic techniques to ensure high purity, often greater than 97% as determined by RP-HPLC and SDS-PAGE .
Biological Functions
SAA1 is involved in several biological processes, including:
- Immune Response: SAA1 plays a crucial role in modulating the immune response. It acts as a chemoattractant for immune cells such as monocytes, neutrophils, and T-lymphocytes .
- Lipid Metabolism: SAA1 is associated with HDL and is involved in the transport and metabolism of cholesterol.
- Inflammation: During acute-phase responses, the levels of SAA1 can increase dramatically, indicating its role in inflammation and tissue repair .
Tissue Distribution
Regulatory Mechanisms
Stability and Storage
Recombinant SAA1 is typically lyophilized and should be stored desiccated below -18°C for long-term stability. Upon reconstitution, it should be stored at 4°C for short-term use and below -18°C for long-term use. It is recommended to add a carrier protein, such as 0.1% HSA or BSA, to prevent freeze-thaw cycles .
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