SAA1 Human
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
The SAA1 is purified by proprietary chromatographic techniques.
Product Specs
Elevated SAA1 levels can lead to secondary amyloidosis, a condition characterized by the extracellular accumulation of amyloid fibrils derived from a circulating precursor. This deposition occurs in various tissues and organs, primarily as a consequence of chronic inflammation, particularly rheumatoid arthritis. In individuals with chronic inflammatory diseases, a cleavage product of SAA1, known as amyloid protein A, deposits systemically as amyloid in vital organs like the liver, spleen, and kidneys. These deposits, highly insoluble and resistant to breakdown, disrupt tissue architecture and impair organ function.
The lyophilization of SAA1 was carried out from a 0.2 µm filtered solution concentrated at 20mM Tris-HCl, pH 9.0 and 150mM NaCl.
Repeated freeze-thaw cycles should be avoided.
(a) RP-HPLC analysis.
(b) SDS-PAGE analysis.
Product Science Overview
Serum Amyloid A (SAA) proteins are a family of apolipoproteins associated with high-density lipoprotein (HDL) in plasma. Among these, Serum Amyloid A1 (SAA1) is a major acute-phase reactant produced predominantly by hepatocytes in response to inflammatory cytokines. The recombinant form of this protein, known as APO-SAA1 (Human Recombinant), is widely used in research to study its biological functions and implications in various diseases.
SAA1 is a small protein consisting of 104 amino acids . It is encoded by the SAA1 gene in humans and is highly conserved across vertebrate species . The protein is primarily synthesized in the liver, although extrahepatic production has also been reported . Under normal physiological conditions, SAA1 is present in the blood at low concentrations (20-50 µg/ml) . However, during the acute-phase response (APR), its levels can increase dramatically, up to 1000-fold within 24 hours .
SAA1 plays a crucial role in the body’s response to inflammation, infection, and trauma. It is involved in several physiological processes, including:
- Lipid Metabolism: SAA1 is associated with HDL and plays a role in lipid transport and metabolism .
- Cytokine-like Activity: SAA1 functions as a cytokine, participating in cell-cell communication and modulating inflammatory and immune responses .
- Acute-Phase Response: SAA1 is a key component of the APR, contributing to the restoration of homeostasis following physiological perturbations .
Elevated levels of SAA1 are associated with various pathological conditions, including chronic inflammatory diseases, infections, and malignancies . It is particularly significant in the diagnosis and monitoring of these conditions, often rising earlier and more sharply than C-reactive protein (CRP), another acute-phase reactant . Additionally, SAA1 has been implicated in the formation of amyloid fibrils, which can accumulate and lead to secondary amyloidosis .
Recombinant human SAA1 (APO-SAA1) is produced using an expression system in E. coli, resulting in a protein with a molecular weight of approximately 11.7 kDa . This recombinant form is used extensively in research to study the protein’s structure, function, and role in disease processes. It is available in a lyophilized form and is highly purified, with a purity of ≥ 98% as determined by SDS-PAGE and HPLC analyses .
