SAA1 Human, His

Serum Amyloid A Human Recombinant (APO-SAA1), His Tag
Cat. No.
BT22144
Source
Escherichia Coli.
Synonyms
Serum amyloid A protein, SAA, Amyloid protein A, Amyloid fibril protein AA, SAA1, SAA2, PIG4, TP53I4, MGC111216.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

SAA1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids (19-122 a.a.) and having a total molecular mass of 13.9 kDa.
SAA1 is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Serum amyloid A1 (SAA1) is an acute-phase protein primarily synthesized by hepatocytes in response to inflammatory signals. Normally present in low levels in the blood, SAA1 plays a role in the immune response, increasing during inflammation to potentially aid tissue repair, combat bacteria, and guide immune cells to infection sites. Additionally, SAA1 acts as an apolipoprotein component of high-density lipoprotein (HDL). However, persistently elevated SAA1 levels can contribute to secondary amyloidosis, a condition characterized by the extracellular accumulation of amyloid fibrils derived from a circulating precursor. This deposition, particularly of the SAA1 cleavage product amyloid protein A, occurs in various tissues and organs, notably the liver, spleen, and kidneys, often as a consequence of chronic inflammation, such as rheumatoid arthritis. The insoluble amyloid deposits disrupt tissue architecture and function, leading to organ impairment.
Description
Recombinant human SAA1, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 125 amino acids (residues 19-122). It has a molecular weight of 13.9 kDa. The protein is engineered with a 20-amino acid His-tag at the N-terminus to facilitate purification, which is achieved using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The SAA1 protein is supplied in a solution containing 20mM Tris buffer at pH 8.0 and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the SAA1 solution can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. The addition of a carrier protein, such as HSA or BSA (0.1%), is advisable for long-term storage. Repeated freezing and thawing of the protein should be avoided.
Purity
The purity of SAA1 is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
Serum amyloid A protein, SAA, Amyloid protein A, Amyloid fibril protein AA, SAA1, SAA2, PIG4, TP53I4, MGC111216.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MRSFFSFLGE AFDGARDMWR AYSDMREANY IGSDKYFHAR GNYDAAKRGP GGVWAAEAIS DARENIQRFF GHGAEDSLAD QAANEWGRSG KDPNHFRPAG LPEKY.

Product Science Overview

Introduction

Serum Amyloid A (SAA) proteins are a family of apolipoproteins associated with high-density lipoprotein (HDL) in plasma. These proteins play a crucial role in the acute phase response, a rapid inflammatory response to trauma, infection, or other stressors. Among the SAA proteins, Serum Amyloid A1 (SAA1) is particularly significant due to its involvement in various physiological and pathological processes.

Structure and Function

SAA1 is a small protein consisting of 104 amino acids . It is primarily produced by hepatocytes in the liver and is secreted into the bloodstream. During an acute phase response, the levels of SAA1 can increase dramatically, up to 1000-fold within 24 hours . This rapid increase is largely due to de novo synthesis in the liver, triggered by inflammatory cytokines .

The recombinant form of SAA1, known as Human Recombinant (APO-SAA1), is often tagged with a His (histidine) tag to facilitate purification and detection. This recombinant protein is produced in E. coli and is a single, non-glycosylated polypeptide chain containing 125 amino acids, including the His tag .

Biological Roles

SAA1 has several important biological functions:

  1. Acute Phase Response: SAA1 is a major acute phase reactant, meaning its levels rise significantly in response to inflammation, infection, or injury .
  2. Lipid Metabolism: SAA1 is associated with HDL and plays a role in lipid transport and metabolism .
  3. Immune Response: SAA1 functions as a cytokine-like protein, involved in cell-cell communication and feedback in inflammatory and immune pathways .
  4. Disease Association: Elevated levels of SAA1 are associated with various diseases, including atherosclerosis, rheumatoid arthritis, and certain cancers .
Applications of Recombinant SAA1

The recombinant form of SAA1 is used in various research and clinical applications:

  1. Biomarker Studies: Due to its role in the acute phase response, SAA1 is studied as a potential biomarker for inflammation and related diseases .
  2. Drug Development: Recombinant SAA1 is used in drug development to understand its role in disease mechanisms and to develop targeted therapies .
  3. Basic Research: Researchers use recombinant SAA1 to study its structure, function, and interactions with other proteins and lipids .

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