RNASE3 Human

Recombinant Human RNASE3 is produced using a mammalian expression system. The target gene encoding RNASE3 spans from Arg28 to Ile160 and is expressed with a 6His tag at the C-terminus . This recombinant protein has a molecular mass of approximately 16.6 kDa under reducing conditions .

RNASE3 exhibits a wide variety of biological activities. It interacts with bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA), demonstrating antibacterial activity. This includes the depolarization of the cytoplasmic membrane of both Gram-negative and Gram-positive bacterial strains . RNASE3 promotes the detachment of the outer membrane of E. coli, alters the overall cell shape, and causes partial loss of cell content .

RNASE3 plays a significant role in the immune response due to its antibacterial properties. It is involved in the body’s defense mechanisms against bacterial infections, particularly those involving eosinophils . The protein’s ability to interact with bacterial components and disrupt their membranes makes it a crucial player in the innate immune system.

Recombinant Human RNASE3 is used extensively in research to study its role in immune responses and its potential therapeutic applications. The protein is supplied in a highly purified form, with a purity greater than 95% as determined by reducing SDS-PAGE . It is typically formulated in a solution containing Tris-HCl, NaCl, DTT, and glycerol, and is stored at temperatures below -70°C to maintain stability .

For optimal stability, RNASE3 should be stored at or below -70°C. It remains stable for up to six months after receipt and for up to three months under sterile conditions after opening. It is important to minimize freeze-thaw cycles to preserve the protein’s integrity .