RNASE2 Human
Sf9, Baculovirus cells.
EDN, RNS2, ribonuclease A family member 2, RAF3, non-secretory ribonuclease, Eosinophil-derived neurotoxin, RNase UpI-2, Ribonuclease 2, RNase 2, Ribonuclease US.
Greater than 90.0% as determined by SDS-PAGE.
Description
RNASE2 Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 143 amino acids (28-161 a.a.) and having a molecular mass of 16.5kDa.
RNASE2 is fused to a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
EDN, RNS2, ribonuclease A family member 2, RAF3, non-secretory ribonuclease, Eosinophil-derived neurotoxin, RNase UpI-2, Ribonuclease 2, RNase 2, Ribonuclease US.
Sf9, Baculovirus cells.
ADPKPPQFTW AQWFETQHIN MTSQQCTNAM QVINNYQRRC KNQNTFLLTT FANVVNVCGN PNMTCPSNKT RKNCHHSGSQ VPLIHCNLTT PSPQNISNCR YAQTPANMFY IVACDNRDQR RDPPQYPVVP VHLDRIIHHH HHH
Product Science Overview
Ribonuclease 2 (RNase 2), also known as eosinophil-derived neurotoxin (EDN), is a member of the RNase A superfamily. This enzyme is primarily produced by eosinophils, a type of white blood cell involved in the body’s immune response. RNase 2 has garnered significant interest due to its diverse biological functions, including its role in immune response and its potential therapeutic applications.
RNase 2 is a small, cationic protein with a molecular weight of approximately 18 kDa. It shares structural similarities with other members of the RNase A superfamily, characterized by a conserved catalytic triad essential for its ribonucleolytic activity. The enzyme cleaves RNA molecules, contributing to the degradation of RNA in various biological processes.
One of the unique features of RNase 2 is its ability to bind to and degrade single-stranded RNA, which is crucial for its antiviral and antimicrobial activities. This enzyme has been shown to possess potent antiviral properties, particularly against respiratory syncytial virus (RSV) and other RNA viruses .
RNase 2 plays a multifaceted role in the immune system. It is stored in the granules of eosinophils and released upon activation. Once released, RNase 2 can exert several effects:
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Antiviral Activity: RNase 2 has been demonstrated to inhibit the replication of various RNA viruses, including RSV. This antiviral activity is attributed to its ability to degrade viral RNA, thereby preventing the virus from replicating and spreading .
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Immune Modulation: RNase 2 can activate dendritic cells (DCs), leading to the production of inflammatory cytokines, chemokines, and growth factors. This activation enhances the immune response by promoting the maturation and activation of DCs, which are essential for initiating adaptive immune responses .
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Alarmin Function: RNase 2 is classified as an alarmin, a type of endogenous molecule released by damaged or stressed cells to alert the immune system. As an alarmin, RNase 2 helps to amplify immune responses and maintain tissue homeostasis .
The recombinant production of RNase 2 involves the expression of the human RNase 2 gene in a suitable host system, such as Escherichia coli or yeast. This process allows for the large-scale production of RNase 2 for research and therapeutic purposes. Recombinant RNase 2 retains the enzymatic and biological properties of the native protein, making it a valuable tool for studying its functions and potential applications.
Given its antiviral and immune-modulating properties, RNase 2 holds promise as a therapeutic agent. Its ability to degrade viral RNA and activate immune cells makes it a potential candidate for the treatment of viral infections and immune-related disorders. Further research is needed to explore its therapeutic potential and develop effective RNase 2-based therapies.
