RNASE3 Human, Sf9
Ribonuclease 3 Human Recombinant, Sf9
Cat. No.
BT14522
Source
Sf9, Baculovirus cells.
Synonyms
ECP, RNS3, Ribonuclease 3, Eosinophil cationic protein, RNASE3, RNASE3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
RNASE3 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 142 amino acids (28-160 a.a.) and having a molecular mass of 16.6kDa (Migrates at 18-28kDa on SDS-PAGE under reducing conditions).
RNASE3 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
RNASE3 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Ribonuclease 3 (RNASE3), also known as eosinophil cationic protein, is a cytotoxic and helminthotoxic protein with relatively weak ribonuclease activity. It exhibits a wide range of biological functions, including antibacterial activity. RNASE3 has been shown to disrupt the cytoplasmic membrane of both Gram-negative and Gram-positive bacteria, leading to depolarization. It can also induce changes in the outer membrane of E. coli, resulting in alterations to cell shape and leakage of cellular contents.
Description
Recombinant human RNASE3, expressed in Sf9 insect cells using a baculovirus system, is a single, glycosylated polypeptide chain comprising 142 amino acids (residues 28-160). It has a molecular weight of 16.6 kDa and appears as a band between 18-28 kDa on SDS-PAGE under reducing conditions. The protein is engineered with a 6-amino acid Histidine tag at the C-terminus to facilitate purification, which is performed using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The RNASE3 protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To further enhance long-term stability during frozen storage, the addition of a carrier protein such as albumin (HSA or BSA) to a final concentration of 0.1% is advised. Avoid repeated cycles of freezing and thawing.
Purity
The purity of RNASE3 is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
ECP, RNS3, Ribonuclease 3, Eosinophil cationic protein, RNASE3, RNASE3.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADPRPPQFTR AQWFAIQHIS LNPPRCTIAM RAINNYRWRC KNQNTFLRTT FANVVNVCGN QSIRCPHNRT LNNCHRSRFR VPLLHCDLIN PGAQNISNCR YADRPGRRFY VVACDNRDPR DSPRYPVVPV HLDTTIHHHH HH.
Product Science Overview
Structure and Production
RNASE3 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 142 amino acids (28-160 a.a.) and has a molecular mass of 16.6 kDa. Under reducing conditions, it migrates at 18-28 kDa on SDS-PAGE . The protein is expressed with a 6 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques .
Biological Activities
RNASE3 exhibits a variety of biological activities, including:
- Antibacterial Activity: RNASE3 has shown antibacterial activity, such as cytoplasmic membrane depolarization of both Gram-negative and Gram-positive strains. It promotes the detachment of the outer membrane of Escherichia coli, alters the overall cell shape, and causes partial loss of cell content .
- Cytotoxicity: The ribonuclease activity of RNASE3 is not essential for its cytotoxicity. Even when the ribonuclease active-site residues are modified to non-functional counterparts, RNASE3 retains its cytotoxicity but loses its ribonuclease activity .
- Helminthotoxicity: RNASE3 is also known for its helminthotoxic properties, making it effective against parasitic infections .
Applications
RNASE3 is used in various research applications due to its diverse biological activities. It is particularly valuable in studies related to:
- Innate Immune Response: RNASE3 plays a role in the innate immune response, especially in mucosal defense against bacteria .
- Inflammation and Asthma: Increased levels of RNASE3 are associated with inflammation and asthma, making it a useful marker in related studies .
- Antimicrobial Research: Its antibacterial and helminthotoxic properties make RNASE3 a subject of interest in antimicrobial research .
Storage and Stability
RNASE3 protein solution (0.5 mg/ml) is formulated with Phosphate Buffered Saline (pH 7.4) and 10% glycerol. For short-term storage, it should be kept at 4°C if used within 2-4 weeks. For long-term storage, it is recommended to store the protein at -20°C with the addition of a carrier protein (0.1% HSA or BSA) to avoid multiple freeze-thaw cycles .
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