RNASE7 Human

Ribonuclease 7 Human Recombinant
Cat. No.
BT14585
Source
Escherichia Coli.
Synonyms
Ribonuclease, RNase A Family, 7, Skin-Derived Antimicrobial Protein 2, RNase 7, SAP-2, EC 3.1.27.5, EC 3.1.27., EC 3.1.27, Ribonuclease 7.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RNASE7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 151 amino acids (29-156 a.a) and having a molecular mass of 16.9kDa.
RNASE7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Ribonuclease 7 (RNASE7) is a member of the RNase A superfamily of ribonucleases. It was first discovered in the stratum corneum of the skin. RNASE7 exhibits potent ribonuclease activity, suggesting its contribution to the notable ribonuclease activity observed in human skin. Studies have demonstrated that RNASE7 possesses a broad spectrum of antimicrobial activity against various pathogenic microorganisms, including remarkably potent activity against specific pathogens.
Description
Recombinant human RNASE7, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 151 amino acids (residues 29-156). It has a molecular weight of 16.9 kDa. The protein is engineered with a 23-amino acid His-tag at the N-terminus to facilitate purification using proprietary chromatographic techniques.
Physical Appearance
Sterile, colorless solution, filtered for purity.
Formulation
RNASE7 protein solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To ensure long-term stability during frozen storage, the addition of a carrier protein like HSA or BSA (0.1%) is advised. Avoid repeated freeze-thaw cycles.
Purity
The purity of RNASE7 is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
Ribonuclease, RNase A Family, 7, Skin-Derived Antimicrobial Protein 2, RNase 7, SAP-2, EC 3.1.27.5, EC 3.1.27., EC 3.1.27, Ribonuclease 7.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSKPKGMTS SQWFKIQHMQ PSPQACNSAM KNINKHTKRC KDLNTFLHEP FSSVAATCQT PKIACKNGDK NCHQSHGPVS LTMCKLTSGK YPNCRYKEKR QNKSYVVACK PPQKKDSQQF HLVPVHLDRV L.

Product Science Overview

Introduction

Ribonuclease 7 (RNase 7) is a member of the Ribonuclease A superfamily, which consists of structurally similar peptides secreted by immune cells and epithelial tissues. RNase 7 is particularly notable for its potent broad-spectrum antimicrobial activity, making it a significant component of the human innate immune system .

Discovery and Source

RNase 7 was initially isolated from the stratum corneum, the outermost layer of human skin . This protein exhibited strong ribonuclease activity, contributing to the overall ribonuclease activity observed in human skin. The recombinant form of RNase 7 is typically produced in Escherichia coli (E. coli) and purified using conventional chromatography techniques .

Structure and Properties

RNase 7 is a cationic peptide, meaning it carries a positive charge, which is crucial for its interaction with negatively charged microbial membranes. The protein consists of 128 amino acids and has a molecular weight of approximately 16.9 kDa . The recombinant version often includes an N-terminal His-tag to facilitate purification and detection .

Antimicrobial Activity

One of the most remarkable features of RNase 7 is its broad-spectrum antimicrobial activity. It has been shown to be effective against a wide range of pathogenic microorganisms, including bacteria, fungi, and viruses . Notably, RNase 7 exhibits potent activity against vancomycin-resistant Enterococcus faecium, a significant concern in clinical settings due to its resistance to multiple antibiotics .

Mechanism of Action

The antimicrobial activity of RNase 7 is primarily attributed to its ability to degrade RNA within microbial cells. By cleaving RNA, RNase 7 disrupts essential cellular processes, leading to the death of the microorganism . Additionally, its cationic nature allows it to interact with and disrupt microbial membranes, further enhancing its antimicrobial efficacy .

Regulation and Expression

RNase 7 is predominantly expressed in epithelial tissues, including the skin, respiratory tract, and urinary tract . Its expression is regulated by various factors, including microbial presence and inflammatory signals. This regulation ensures that RNase 7 is produced in response to infection, providing a rapid and effective defense mechanism .

Potential Therapeutic Applications

Given its potent antimicrobial properties, RNase 7 holds promise as a novel therapeutic agent for treating infections, particularly those caused by antibiotic-resistant bacteria . Further research is needed to fully understand its potential and to develop effective delivery methods for clinical use.

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