RHOD Human
Rho-related GTP-binding protein RhoD, Rho-related protein HP1, RhoHP1, RHOD, ARHD, Rho, RHOM.
Description
RHOD produced in E.Coli is a single, non-glycosylated polypeptide chain containing 211 amino acids (18-207 a.a.) and having a molecular mass of 23.8kDa.
RHOD is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Rho-related GTP-binding protein RhoD, Rho-related protein HP1, RhoHP1, RHOD, ARHD, Rho, RHOM.
MGSSHHHHHH SSGLVPRGSH MVKVVLVGDG GCGKTSLLMV FADGAFPESY TPTVFERYMV NLQVKGKPVH LHIWDTAGQD DYDRLRPLFY PDASVLLLCF DVTSPNSFDN IFNRWYPEVN HFCKKVPIIV VGCKTDLRKD KSLVNKLRRN GLEPVTYHRG QEMARSVGAV AYLECSARLH
DNVHAVFQEA AEVALSSRGR NFWRRITQGF C.
Product Science Overview
Ras Homolog Gene Family Member D (RHOD) is a protein encoded by the RHOD gene in humans. It belongs to the Rho family of GTPases, which are part of the larger Ras superfamily of small GTP-binding proteins. These proteins play crucial roles in various cellular processes, including cytoskeletal dynamics, cell migration, and intracellular trafficking.
The RHOD gene is located on chromosome 11 and encodes a protein that is approximately 21 kDa in size. The RHOD protein contains a GTPase domain, which is responsible for binding and hydrolyzing GTP. This domain is highly conserved among members of the Rho family, indicating its importance in the protein’s function .
RHOD is involved in several cellular processes, primarily related to the actin cytoskeleton and membrane trafficking. It plays a significant role in endosome dynamics, which are essential for the internalization and trafficking of activated tyrosine kinase receptors such as the platelet-derived growth factor receptor beta (PDGFRB) .
One of the key functions of RHOD is to coordinate membrane transport with the function of the cytoskeleton. This coordination is crucial for maintaining cellular structure and facilitating various cellular activities, including cell division and migration .
RHOD interacts with several other proteins to carry out its functions. It has been shown to interact with WHAMM (WASP Homolog Associated with Actin, Golgi Membranes, and Microtubules), which is involved in the regulation of actin filament bundling and filopodia formation . Additionally, RHOD can modulate the effect of DAPK3 (Death-Associated Protein Kinase 3) in the reorganization of the actin cytoskeleton and focal adhesion dissolution .
Mutations or dysregulation of RHOD have been associated with various diseases. For instance, alterations in RHOD expression have been linked to Wiskott-Aldrich Syndrome and Anal Canal Squamous Cell Carcinoma . Understanding the role of RHOD in these diseases could provide insights into potential therapeutic targets.
Research on RHOD continues to uncover its diverse roles in cellular processes and its potential implications in disease. The recombinant form of RHOD is used in various experimental settings to study its function and interactions with other proteins. These studies are crucial for developing targeted therapies for diseases associated with RHOD dysregulation.
