RHOA Human
Description
The RHOA is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The Ras Homolog Gene Family Member A (RhoA) is a protein encoded by the RHOA gene. It belongs to the Rho family of small GTPases, which are known to act as molecular switches in various signal transduction pathways. These proteins play a crucial role in the regulation of the actin cytoskeleton, influencing cell shape, attachment, and motility .
RhoA cycles between an inactive GDP-bound state and an active GTP-bound state. In its active form, RhoA interacts with a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration, and cell cycle progression . It is involved in a signal transduction pathway that links plasma membrane receptors to the assembly of focal adhesions and actin stress fibers .
RhoA is essential for several cellular processes:
- Cytoskeleton Organization: RhoA is primarily associated with the organization of the cytoskeleton. It regulates the formation of actin stress fibers and focal adhesions, which are critical for maintaining cell shape and enabling cell movement .
- Cell Cycle and Cytokinesis: RhoA plays a vital role in cell cycle progression and cytokinesis. It is involved in the formation of the myosin contractile ring during cell division, which is necessary for the successful completion of cytokinesis .
- Cell Migration and Adhesion: RhoA regulates cell migration by controlling the dynamics of the actin cytoskeleton. It is also required for the formation of apical junctions in keratinocyte cell-cell adhesion .
Overexpression of RhoA has been associated with tumor cell proliferation and metastasis. It is implicated in various cancers due to its role in promoting cell cycle progression and migration . Additionally, RhoA has been studied in the context of cardiac pathophysiology, where it has been shown to play a role in cardiac remodeling and cardiomyopathies .
Human recombinant RhoA is a form of the protein that is produced through recombinant DNA technology. This involves inserting the RHOA gene into an expression system, such as bacteria or yeast, to produce the protein in large quantities. Recombinant RhoA is used in research to study its function and role in various cellular processes and diseases.
