RHOB Human
Ras Homolog Gene Family Member B Human Recombinant
Cat. No.
BT10866
Source
Escherichia Coli.
Synonyms
Rho-related GTP-binding protein RhoB, Rho cDNA clone 6, h6, RHOB, ARH6, ARHB, RHOH6, MST081, MSTP081.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
RHOB produced in E.Coli is a single, non-glycosylated polypeptide chain containing 213 amino acids (1-193 a.a.) and having a molecular mass of 23.9kDa (Molecular weight on SDS-PAGE will appear higher).
RHOB is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
RHOB is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
RhoB, a member of the Rho GTP-binding protein family, is involved in apoptosis regulation in cells undergoing neoplastic transformation after DNA damage. Notably, RhoB acts as a tumor suppressor, with its deletion potentially leading to tumor development.
Description
This Escherichia coli-derived RHOB protein is a single, non-glycosylated polypeptide chain with a molecular weight of 23.9 kDa. It consists of 213 amino acids, spanning positions 1 to 193, and includes a 20 amino acid His-tag fused at the N-terminus. Purification is achieved using proprietary chromatographic techniques, resulting in a highly purified protein.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The RHOB protein is supplied in a solution at a concentration of 0.25 mg/ml. The formulation buffer consists of 20 mM Tris-HCl (pH 8.0), 2 mM DTT, 20% glycerol, and 0.2 mM NaCl.
Stability
For short-term storage (2-4 weeks), the protein solution should be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein such as HSA or BSA (0.1%). It is important to avoid repeated cycles of freezing and thawing.
Purity
The purity of the RHOB protein is determined to be greater than 90% based on SDS-PAGE analysis.
Synonyms
Rho-related GTP-binding protein RhoB, Rho cDNA clone 6, h6, RHOB, ARH6, ARHB, RHOH6, MST081, MSTP081.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAAIRKKLVV VGDGACGKTC LLIVFSKDEF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWV PEVKHFCPNV PIILVANKKD LRSDEHVRTE LARMKQEPVR TDDGRAMAVR IQAYDYLECS AKTKEGVREV FETATRAALQ KRYGSQNGCI NCC.
Product Science Overview
Introduction
Gene and Protein Structure
The RHOB gene is located on chromosome 2 in humans and encodes a protein that is approximately 21 kDa in size. The protein consists of 196 amino acids and has a high degree of homology with other members of the Rho family, such as RHOA and RHOC . The RHOB protein contains several conserved domains, including the GTP-binding domain, which is essential for its function as a molecular switch .
Function and Mechanism
RHOB plays a crucial role in regulating the actin cytoskeleton, which is important for maintaining cell shape, motility, and division . It is involved in various cellular processes, including:
- Cell Migration: RHOB regulates the dynamics of the actin cytoskeleton, which is essential for cell movement .
- Cell Adhesion: It influences the formation of focal adhesions, which are contact points between cells and the extracellular matrix .
- Vesicle Trafficking: RHOB is involved in the transport of vesicles within cells, particularly in the endocytic pathway .
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
