RHOC Human

Ras Homolog Gene Family Member C Human Recombinant
Cat. No.
BT10934
Source
Escherichia Coli.
Synonyms
ARH9, ARHC, H9, RHOH9, RAS-related homolog 9, Rho cDNA clone 9, Rho-related GTP-binding protein RhoC, ARH9, MGC1448, MGC61427, RHOC.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RHOC Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 210 amino acids (1-190 a.a.) and having a molecular mass of 23.8 kDa. The RHOC is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
RHOC, a member of the Rac subfamily within the Rho family of GTPases, is a small signaling G protein/GTPase. It acts as a molecular switch in signal transduction cascades, cycling between inactive GDP-bound and active GTP-bound states. RHOC plays a crucial role in reorganizing the actin cytoskeleton, influencing cell shape, adhesion, and movement.
Description
Recombinant Human RHOC, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 210 amino acids (1-190 a.a.) with a molecular weight of 23.8 kDa. It features a 20 amino acid His-Tag at the N-terminus and undergoes purification via proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The RHOC Human solution is provided at a concentration of 0.5mg/ml and contains 20mM Tris-HCl (pH 8), 0.1M NaCl, 1mM DTT, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
SDS-PAGE analysis indicates a purity greater than 90.0%.
Synonyms
ARH9, ARHC, H9, RHOH9, RAS-related homolog 9, Rho cDNA clone 9, Rho-related GTP-binding protein RhoC, ARH9, MGC1448, MGC61427, RHOC.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYIADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT PEVKHFCPNV PIILVGNKKD LRQDEHTRRE LAKMKQEPVR SEEGRDMANR ISAFGYLECS AKTKEGVREV FEMATRAGLQ VRKNKRRRGC.

Product Science Overview

Introduction

Ras Homolog Gene Family Member C (RHOC) is a small signaling G protein, specifically a GTPase, and a member of the Rac subfamily within the Rho family of GTPases . The RHOC gene encodes this protein, which plays a crucial role in various cellular processes, including cell shape, attachment, and motility . RHOC is known for its involvement in the reorganization of the actin cytoskeleton and regulation of cell locomotion .

Gene and Protein Structure

The RHOC gene is located on chromosome 1 (1p13.2) in humans . The protein encoded by this gene is approximately 21 kDa in size and is prenylated at its C-terminus, allowing it to localize to the cytoplasm and plasma membrane . RHOC cycles between inactive GDP-bound and active GTP-bound states, functioning as a molecular switch in signal transduction cascades .

Biological Functions

RHOC is essential for various biological processes, including:

  • Cell Motility: RHOC promotes cell movement by reorganizing the actin cytoskeleton .
  • Signal Transduction: It acts as a molecular switch in signaling pathways, linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers .
  • Cytokinesis: RHOC is required for the formation of the myosin contractile ring during cell cycle cytokinesis .
  • Tumor Progression: Overexpression of RHOC is associated with tumor cell proliferation and metastasis, making it a significant factor in cancer research .
Clinical Significance

RHOC has been implicated in various diseases, particularly in cancer. Its overexpression is linked to breast disease and inflammatory breast carcinoma . Due to its role in cell motility and invasion, RHOC is a target for therapeutic interventions aimed at inhibiting tumor metastasis .

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