Rantes Rhesus Macaque

Rantes Rhesus Macaque Recombinant (CCL5)
Cat. No.
BT23143
Source
Escherichia Coli.
Synonyms
C-C motif chemokine 5, Small-inducible cytokine A5, T-cell-specific protein RANTES, CCL5.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Rantes Rhesus Macaque Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 68 amino acids and having a molecular mass of 7.8kDa.
The CCL5 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
RANTES, also known as CCL5, is an 8 kDa chemokine that plays a crucial role in immune responses. It attracts and activates leukocytes, particularly T cells, eosinophils, and basophils, to inflammatory sites. RANTES also contributes to the activation of natural killer (NK) cells, leading to the formation of CHAK (CC-Chemokine-activated killer) cells. Notably, it exhibits HIV-suppressive properties. In humans, the gene encoding RANTES is located on chromosome 17.
Description
This product consists of the recombinant form of Rhesus Macaque Rantes, produced in E. coli. It is a single, non-glycosylated polypeptide chain with 68 amino acids and a molecular weight of 7.8 kDa. The purification process involves proprietary chromatographic techniques.
Physical Appearance
The product appears as a sterile, white, lyophilized powder.
Formulation
The Rantes protein was lyophilized from a 0.2 µm filtered solution containing 20mM PB (pH 6.0) and 500mM NaCl.
Solubility
To reconstitute the lyophilized Rantes, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. Further dilutions can be made using other aqueous solutions.
Stability
Lyophilized Rantes remains stable at room temperature for up to 3 weeks. However, for extended storage, it is advisable to store it in a desiccated state below -18°C. Once reconstituted, CCL5 should be stored at 4°C for 2-7 days. For long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles.
Purity
The purity of the product exceeds 98.0%, as determined by RP-HPLC and SDS-PAGE analyses.
Biological Activity
The biological activity of the product, as assessed by a chemotaxis bioassay using human peripheral blood monocytes, falls within the concentration range of 1.0-10 ng/ml.
Synonyms
C-C motif chemokine 5, Small-inducible cytokine A5, T-cell-specific protein RANTES, CCL5.
Source
Escherichia Coli.
Amino Acid Sequence
SPHASDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS.

Product Science Overview

Introduction

RANTES (Regulated upon Activation, Normal T-cell Expressed and Secreted), also known as CCL5 (C-C motif chemokine ligand 5), is a chemokine that plays a crucial role in the immune system by attracting immune cells to sites of inflammation. This protein is part of the beta-chemokine family and is involved in various immune responses, including the recruitment of T cells, eosinophils, and basophils.

Structure and Function

RANTES is an 8 kDa protein that is classified as a chemotactic cytokine or chemokine . It is known for its ability to attract and activate leukocytes, which are white blood cells involved in the body’s immune response . The protein is produced by various cell types, including T cells, platelets, and certain types of epithelial cells.

Recombinant RANTES

Recombinant RANTES refers to the protein that has been produced through recombinant DNA technology. This involves inserting the gene that encodes RANTES into a suitable expression system, such as E. coli, to produce the protein in large quantities. Recombinant RANTES is used in research to study its role in immune responses and to develop potential therapeutic applications.

Rhesus Macaque RANTES

The Rhesus Macaque (Macaca mulatta) is a species of monkey that is often used in biomedical research due to its close genetic relationship to humans. Rhesus Macaque RANTES is the version of the protein that is derived from this species. It shares a high degree of similarity with human RANTES, making it a valuable tool for studying the protein’s function in a model organism that closely mimics human physiology.

Applications in Research

Recombinant Rhesus Macaque RANTES is used in various research applications, including:

  • Chemoattraction Studies: Researchers use recombinant RANTES to study its ability to attract immune cells, such as T cells and eosinophils, to sites of inflammation .
  • Immune Response Research: The protein is used to investigate the mechanisms by which RANTES contributes to immune responses and its role in diseases characterized by inflammation .
  • Therapeutic Development: Recombinant RANTES is being explored as a potential therapeutic agent for conditions involving immune dysregulation, such as autoimmune diseases and chronic inflammatory conditions .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

Rantes Human
Rantes Human Recombinant (CCL5)
Cat. No.
BT22732
Source
Escherichia Coli.
Rantes Human, His
Rantes Human Recombinant (CCL5), His Tag
Cat. No.
BT22829
Source
Escherichia Coli.
Rantes Mouse
Rantes Mouse Recombinant (CCL5)
Cat. No.
BT22923
Source
Escherichia Coli.
Rantes Rat
Rantes Rat Recombinant (CCL5)
Cat. No.
BT23055
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.