Rantes Rat

Rantes Rat Recombinant (CCL5)
Cat. No.
BT23055
Source
Escherichia Coli.
Synonyms
Small inducible cytokine A5, CCL5, T-cell-specific RANTES protein, SIS-delta, T cell- specific protein P228, TCP228, chemokine (C-C motif) ligand 5, SISd, SCYA5, RANTES, D17S136E, MGC17164.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Rantes Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 68 amino acids and having a molecular mass of 7876 Dalton.
The Rat Rantes is purified by proprietary chromatographic techniques.

Product Specs

Introduction
RANTES, also known as CCL5, is an 8 kDa chemokine that plays a crucial role in immune responses. It attracts leukocytes like T cells, eosinophils, and basophils to inflammation sites. RANTES also contributes to the activation and proliferation of specific natural killer (NK) cells, leading to the formation of CHAK (CC-Chemokine-activated killer) cells. This chemokine, found on chromosome 17 in humans, also exhibits HIV-suppressive properties.
Description
Recombinant Rat Rantes, produced in E. coli, is a non-glycosylated polypeptide chain composed of 68 amino acids, with a molecular weight of 7876 Daltons. It is purified using proprietary chromatographic methods to ensure high purity.
Physical Appearance
White, sterile-filtered powder obtained through lyophilization (freeze-drying).
Formulation
Lyophilized from a 1 mg/ml solution in water, without any additives.
Solubility
To reconstitute lyophilized Rantes, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can be further diluted with other aqueous solutions as needed.
Stability
Lyophilized Rat Rantes remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated at a temperature below -18°C. Once reconstituted, Rat CCL5 should be stored at 4°C for 2-7 days. For extended storage, adding a carrier protein (0.1% HSA or BSA) is advisable. Avoid repeated freeze-thaw cycles.
Purity
The purity of Rat Rantes is greater than 95%, as determined by Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) and SDS-PAGE analysis.
Biological Activity
The biological activity is measured by the ability to attract human lymphocytes and murine T-cells. The effective concentration range is between 1-10 ng/ml, which corresponds to a specific activity of 100,000-1,000,000 IU/mg.
Synonyms
Small inducible cytokine A5, CCL5, T-cell-specific RANTES protein, SIS-delta, T cell- specific protein P228, TCP228, chemokine (C-C motif) ligand 5, SISd, SCYA5, RANTES, D17S136E, MGC17164.
Source
Escherichia Coli.
Amino Acid Sequence
SPYGSDTTPC CFAYLSLALP RAHVKEYFYT SSKCSNLAVV FVTRRNRQVC ANPEKKWVQE YINYLEMS.

Product Science Overview

Structure and Function

RANTES is an 8 kDa protein that is chemotactic for T cells, eosinophils, and basophils . It is known for its ability to recruit leukocytes to sites of inflammation, which is essential for the body’s defense mechanisms against infections and injuries . The protein achieves this by binding to specific receptors on the surface of target cells, such as CCR1, CCR3, and CCR5 .

Production and Regulation

RANTES is produced by various cell types, including T cells, macrophages, and platelets. Its expression is regulated by inflammatory cytokines and other signaling molecules. Upon activation, these cells secrete RANTES, which then participates in the recruitment of immune cells to the site of inflammation .

Recombinant RANTES

Recombinant RANTES, such as the rat recombinant version, is produced using genetic engineering techniques. This involves inserting the gene encoding RANTES into a suitable expression system, such as E. coli, to produce the protein in large quantities . Recombinant RANTES is used in research to study its role in immune responses and to develop potential therapeutic applications.

Applications in Research and Medicine

RANTES has been extensively studied for its role in various diseases, including autoimmune disorders, cancer, and infectious diseases. Its ability to attract and activate immune cells makes it a potential target for therapeutic interventions. For example, blocking RANTES activity could help reduce inflammation in autoimmune diseases, while enhancing its activity could boost immune responses against infections and tumors .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

Rantes Human
Rantes Human Recombinant (CCL5)
Cat. No.
BT22732
Source
Escherichia Coli.
Rantes Human, His
Rantes Human Recombinant (CCL5), His Tag
Cat. No.
BT22829
Source
Escherichia Coli.
Rantes Mouse
Rantes Mouse Recombinant (CCL5)
Cat. No.
BT22923
Source
Escherichia Coli.
Rantes Rhesus Macaque
Rantes Rhesus Macaque Recombinant (CCL5)
Cat. No.
BT23143
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.