RANBP3 Human
RAN Binding Protein 3 Human Recombinant
Cat. No.
BT2366
Source
Escherichia Coli.
Synonyms
RAN binding protein 3, RanBP3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
RANBP3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 234 amino acids (235-445 a.a) and having a molecular mass of 25.5kDa.
RANBP3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
RANBP3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
RAN Binding Protein 3, also known as RANBP3, is a protein found in both the nucleus and cytoplasm of cells. It plays a crucial role in nuclear export, a process by which molecules are transported from the nucleus to the cytoplasm. This function is facilitated by RANBP3's RanBD1 domain, which allows it to interact with other proteins and form a complex that mediates nuclear export. In addition to its role in nuclear transport, RANBP3 also acts as a negative regulator of TGF-beta signaling. TGF-beta signaling is a cellular pathway involved in various cellular processes, including cell growth, differentiation, and apoptosis. RANBP3 regulates this pathway by binding to and inhibiting the activity of R-SMAD proteins, specifically SMAD2 and SMAD3, which are key downstream effectors of the TGF-beta pathway.
Description
This product consists of the human RANBP3 protein, recombinantly produced in E. coli bacteria. This protein is not glycosylated and exists as a single polypeptide chain composed of 234 amino acids (specifically, amino acids 235-445 of the full-length protein). It has a molecular weight of 25.5 kDa. For purification and detection purposes, a 23-amino acid His-tag is fused to the N-terminus of the protein. The protein is purified using proprietary chromatographic techniques to ensure its high purity.
Physical Appearance
The product is a clear, colorless solution that has been sterilized by filtration.
Formulation
The RANBP3 protein is supplied in a solution with a concentration of 0.5 mg/ml. The solution also contains 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl (sodium chloride), and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to freeze the product at -20°C. To prevent protein degradation due to repeated freezing and thawing, it is advisable to aliquot the product into smaller portions before freezing. For optimal long-term storage, adding a carrier protein like HSA (human serum albumin) or BSA (bovine serum albumin) at a concentration of 0.1% is recommended. This helps to stabilize the protein and prevent its degradation.
Purity
The purity of the RANBP3 protein in this product is greater than 95%, as determined by SDS-PAGE analysis. This technique separates proteins based on their size, allowing for the assessment of protein purity.
Synonyms
RAN binding protein 3, RanBP3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSISSSLEN STNSADASSN KFVFGQNMSE RVLSPPKLNE VSSDANRENA AAESGSESSS QEATPEKESL AESAAAYTKA TARKCLLEKV EVITGEEAES NVLQMQCKLF VFDKTSQSWV ERGRGLLRLN DMASTDDGTL QSRLVMRTQG SLRLILNTKL WAQMQIDKAS EKSIRITAMD TEDQGVKVFL ISASSKDTGQ LYAALHHRIL ALRS.
Product Science Overview
Structure and Function
RANBP3 contains a RanBD1 domain and is found in both the nucleus and cytoplasm. It functions as a scaffolding protein, stabilizing the interaction between XPO1/CRM1 and its cargo . In the absence of Ran-bound GTP, RANBP3 prevents the binding of XPO1/CRM1 to the nuclear pore complex . Additionally, it binds to CHC1/RCC1 and enhances the guanine nucleotide exchange activity of CHC1/RCC1 .
Role in Cellular Processes
RANBP3 is involved in several cellular processes, including:
- Nuclear Export: It recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner, facilitating the nuclear export of proteins .
- Regulation of TGF-beta Signaling: RANBP3 interacts with R-SMAD proteins, SMAD2 and SMAD3, mediating their nuclear export and acting as a negative regulator of TGF-beta signaling .
- Mitotic Spindle Assembly: The protein plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules .
Clinical Significance
Research and Applications
Human recombinant RANBP3 is used in research to study its function and interactions with other proteins. It is also utilized in assays to investigate its role in nuclear export and signaling pathways.
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