RANBP1 Human

RAN Binding Protein 1 Human Recombinant
Cat. No.
BT2261
Source
E.coli.
Synonyms
RAN binding protein 1, HTF9A, ran-specific GTPase-activating protein, RanBP1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RANBP1 Human Recombinant produced in E. coli is a single polypeptide chain containing 225 amino acids (1-201) and having a molecular mass of 25.8kDa (molecular size on SDS-PAGE will appear higher).
RANBP1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RANBP1 belongs to a family of proteins that interact with Ran GTPase. Ran GTPase plays a crucial role in various cellular processes, including cell cycle regulation, mRNA processing, nuclear transport, mitotic spindle assembly, postmitotic nuclear reassembly, and the maintenance of nuclear architecture. While RANBP1 itself does not stimulate the GTPase activity of Ran, it significantly enhances GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). Furthermore, RANBP1 acts as a negative regulator of RCC1 by suppressing RCC1-mediated guanine nucleotide release from Ran.
Description
Recombinant human RANBP1, produced in E. coli, is a single polypeptide chain comprising 225 amino acids (residues 1-201) with a molecular weight of 25.8 kDa. Note that the molecular size observed on SDS-PAGE will appear larger. This RANBP1 protein is fused to a 24 amino acid His-tag at its N-terminus and purified using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The RANBP1 solution is provided at a concentration of 1 mg/ml in a buffer composed of 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the RANBP1 solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the RANBP1 protein is determined to be greater than 95% by SDS-PAGE analysis.
Synonyms
RAN binding protein 1, HTF9A, ran-specific GTPase-activating protein, RanBP1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAAAKD THEDHDTSTE NTDESNHDPQ FEPIVSLPEQ EIKTLEEDEE ELFKMRAKLF RFASENDLPE WKERGTGDVK LLKHKEKGAI RLLMRRDKTL KICANHYITP MMELKPNAGS DRAWVWNTHA DFADECPKPE LLAIRFLNAE NAQKFKTKFE ECRKEIEERE KKAGSGKNDH AEKVAEKLEA LSVKEETKED AEEKQ.

Product Science Overview

Function and Mechanism

RANBP1 forms a complex with Ras-related nuclear protein (Ran) and metabolizes guanoside triphosphate (GTP). This complex is essential for regulating the cell cycle by controlling the transport of proteins and nucleic acids into the nucleus . RANBP1 specifically interacts with GTP-charged RAN and does not activate the GTPase activity of RAN but significantly increases GTP hydrolysis by the RanGTPase-activating protein (RANGAP1) .

Role in Cellular Processes

RANBP1 is involved in several critical cellular processes, including:

  • Regulation of the Cell Cycle: By controlling the transport of proteins and nucleic acids into the nucleus, RANBP1 plays a vital role in cell cycle regulation .
  • Mitotic Regulation: RANBP1 has been implicated as a mitotic regulator of RCC1 (Regulator of Chromatin Condensation 1) in embryonic systems .
  • GTP-RAN and GDP-RAN Fluctuations: RANBP1 regulates the activity of RANGAP1, thus controlling the fluctuations between GTP-RAN and GDP-RAN .
Associated Diseases

Mutations or dysregulation of the RANBP1 gene have been associated with various diseases, including:

  • Hematologic Cancer: Abnormalities in RANBP1 expression have been linked to certain types of hematologic cancers .
  • Salt and Pepper Syndrome: This rare genetic disorder has also been associated with mutations in the RANBP1 gene .
Research and Applications

RANBP1 continues to be a subject of extensive research due to its critical role in cellular processes and its potential implications in various diseases. The recombinant form of RANBP1 is used in research to study its function and mechanism in detail, providing insights into its role in health and disease.

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