PTHrP N15 Human
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
The PTHrP is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Parathyroid Hormone Related Protein (PTHrP) is a critical regulator of calcium homeostasis and bone metabolism. The N15 labeled version of this protein, produced recombinantly in E. coli, is a valuable tool for research and clinical applications due to its stable isotope labeling, which allows for precise tracking and quantification in various biological systems.
PTHrP N15 Human Recombinant is a single, non-glycosylated polypeptide chain containing 86 amino acids, with a molecular weight of approximately 10,033 Da . The protein is labeled with the stable isotope N15, which is incorporated during its production in E. coli. This labeling is achieved through proprietary chromatographic techniques that ensure high purity and stability of the protein .
PTHrP plays a multifaceted role in the body, primarily involved in the regulation of calcium levels. It acts on various tissues, including bones, kidneys, and the intestines, to modulate calcium absorption, reabsorption, and release. In bones, PTHrP enhances the release of calcium by stimulating osteoclasts indirectly through osteoblasts . In the kidneys, it promotes the reabsorption of calcium from the distal tubules and the thick ascending limb . In the intestines, PTHrP increases calcium absorption by upregulating the production of active vitamin D .
The N15 labeled PTHrP is particularly useful in research settings where precise quantification and tracking of the protein are required. Its stable isotope labeling allows researchers to study the protein’s dynamics, interactions, and metabolic pathways with high accuracy. This is crucial for understanding the protein’s role in various physiological and pathological conditions, including osteoporosis, hypercalcemia, and certain cancers.
The lyophilized form of PTHrP N15 is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage . Upon reconstitution, the protein should be stored at 4°C for short-term use and below -18°C for long-term use, with the addition of a carrier protein to prevent freeze-thaw cycles .
