PTH (1-34) Human

Parathyroid hormone (PTH), also known as parathormone, is an 84-amino acid polypeptide hormone secreted by the parathyroid glands. It plays a crucial role in calcium homeostasis by increasing calcium levels in the bloodstream, in contrast to calcitonin, a hormone produced by the thyroid gland that lowers calcium levels. PTH exerts its calcium-elevating effects by targeting receptors in bones, kidneys, and the intestine. In bones, PTH stimulates the release of calcium from bone reserves through a process called bone resorption. This process involves the activation of osteoclasts, cells responsible for bone breakdown. PTH indirectly stimulates osteoclasts by binding to osteoblasts, the bone-forming cells. This binding leads to increased expression of RANKL by osteoblasts, which then interacts with RANK receptors on osteoclast precursors, promoting their fusion and activation. In the kidneys, PTH enhances the active reabsorption of calcium from the distal tubules and the thick ascending limb. In the intestine, PTH indirectly promotes calcium absorption by stimulating the production of active vitamin D (1,25-dihydroxy vitamin D). PTH achieves this by upregulating the enzyme responsible for the 1-alpha hydroxylation of 25-hydroxy vitamin D, the precursor to the active form. Active vitamin D then enhances calcium absorption in the intestine through the involvement of calbindin. Recombinant human full-length PTH (1-84) holds promise as a potential treatment for osteoporosis. Its bone formation-stimulating properties stem from its ability to increase bone turnover by stimulating osteoblasts, thereby reducing both vertebral and non-vertebral fractures.

Synthetic human parathyroid hormone (C₁₈₁H₂₉₁N₅₅O₅₁S₂) is a peptide composed of 34 amino acids with a molecular weight of 4117.72 Daltons.
The purification of PTH is achieved using proprietary chromatographic methods.

White, sterile-filtered lyophilized powder.

The protein was lyophilized at a concentration of 1 mg/ml without any additional ingredients.

To reconstitute the lyophilized parathyroid hormone, it is recommended to dissolve it in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.

Lyophilized parathyrin remains stable at room temperature for up to 3 weeks. However, for extended storage, it is recommended to store it in a desiccated state below -18°C. Once reconstituted, PTH should be stored at 4°C and used within 2-7 days. For long-term storage, consider adding a carrier protein such as 0.1% HSA or BSA. Avoid repeated freeze-thaw cycles.

The purity is determined to be greater than 99.0% as measured by RP-HPLC.

Parathyrin, PTH, Parathormone.

H-Ser-Val-Ser-Glu-Ile-Gln-Leu-Met-His-Asn-Leu-Gly-Lys-His-Leu-Asn-Ser-Met-Glu-Arg-Val-Glu-Trp-Leu-Arg-Lys-Lys-Leu-Gln-Asp-Val-His-Asn-Phe-OH.