PTH (1-84) Human

Parathyroid hormone (PTH), also known as parathormone, is an 84-amino acid polypeptide secreted by the parathyroid glands. It functions to elevate calcium levels in the bloodstream, in contrast to calcitonin (produced by the thyroid gland's parafollicular cells) which lowers calcium concentration. PTH increases blood calcium levels by targeting parathyroid hormone receptors in three key areas: 1. **Bones:** PTH promotes calcium release from bone reserves. This process, called bone resorption, involves the breakdown of bone by osteoclasts, which are indirectly stimulated by PTH. Osteoclasts lack PTH receptors; instead, PTH binds to osteoblasts (bone-forming cells), prompting them to increase RANKL expression. RANKL binds to RANK receptors on osteoclast precursors, triggering their fusion into mature osteoclasts, thus enhancing bone resorption. 2. **Kidneys:** PTH enhances active calcium reabsorption in the distal tubules and thick ascending limb of the kidneys. 3. **Intestines:** PTH boosts intestinal calcium absorption by increasing vitamin D production and upregulating the enzyme responsible for converting vitamin D to its active form (1,25-dihydroxy vitamin D) through 1-alpha hydroxylation of 25-hydroxy vitamin D. Active vitamin D facilitates calcium absorption (as Ca2+ ions) in the intestine via calbindin. Recombinant Human full-length PTH 1-84 shows potential as an anti-osteoporotic agent. Its bone formation-stimulating properties increase bone turnover, stimulating osteoblasts and reducing both vertebral and non-vertebral fractures.

Parathyroid Hormone 1-84 (full length) Human Recombinant, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 84 amino acids, with a molecular weight of approximately 9.4 kDa. The PTH is purified using proprietary chromatographic techniques.

Sterile Filtered White Lyophilized (Freeze-Dried) Powder

Lyophilized from a 0.2 μm filtered concentrated solution in phosphate-buffered saline (PBS) at pH 7.4.

Reconstitute the lyophilized Parathormone in sterile 18 MΩ-cm H2O at a concentration of at least 100 μg/ml. Further dilutions can be made in other aqueous solutions.

Lyophilized Parathyrin remains stable at room temperature for 3 weeks but should be stored desiccated below -18°C. Upon reconstitution, store PTH at 4°C for 2-7 days. For long-term storage, freeze at -18°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid freeze-thaw cycles.

Greater than 95.0% as determined by: (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis

The activity is determined by its capacity to induce cAMP accumulation in murine MC3T3E1 cells and is <50 ng/ml, corresponding to a specific activity exceeding 2.0 x 10⁴ Units/mg.

Parathyrin, PTH, Parathormone.

Escherichia Coli.

SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNFVALGAP LAPRDAGSQR PRKKEDNVLV ESHEKSLGEA DKADVNVLTK AKSQ.